Influence of Krypton Gas Nanobubbles on the Activity of Pepsin
文献类型：期刊论文
| 作者 | Wang, J; Yuan, KW; Wang, XY; Zhang, LJ; Hu, J |
| 刊名 | LANGMUIR
 |
| 出版日期 | 2020 |
| 卷号 | 36期号:46页码:14070-14075 |
| ISSN号 | 0743-7463 |
| DOI | 10.1021/acs.langmuir.0c02635 |
| 文献子类 | 期刊论文 |
| 英文摘要 | The fact that biologically inert gases can significantly affect the biological function of proteins still lacks a full understanding because they are usually chemically stable and weakly absorbed by biological molecules. Recently, nanobubbles were proposed to play an important role in the activity of a protein Scientific reports 2013, 3; Scientific reports 2017, 7, 10176). In this study, we developed a controllable method to produce high-concentration krypton (Kr) gas nanobubbles in pure water and measured the concentration influence of those Kr nanobubbles on pepsin protein activity. By combining high-sensitivity synchrotron radiation X-ray fluorescence techniques with a nanoparticle tracking analysis technology, we provided strong evidence that the observed "nanoparticles" were indeed Kr nanobubbles. Activity measurements showed that the activity would be inhibited by the existence of Kr nanobubbles and could be recovered by degassing. More importantly, the inhibition extent of pepsin activity was dominated by the number of nanobubbles in solution. More nanobubbles would cause more inhibition of pepsin activity. Furthermore, the structures of pepsin could be changed by nanobubbles, which might be the reason for inhabitation of activity. Our results would provide a further understanding of the mechanisms of the biological effects of inert gases. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32938]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Adv Res Inst, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Key Lab Interfacial Phys & Technol, Shanghai 201800, Peoples R China 3.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201204, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, J,Yuan, KW,Wang, XY,et al. Influence of Krypton Gas Nanobubbles on the Activity of Pepsin[J]. LANGMUIR,2020,36(46):14070-14075. |
| APA | Wang, J,Yuan, KW,Wang, XY,Zhang, LJ,&Hu, J.(2020).Influence of Krypton Gas Nanobubbles on the Activity of Pepsin.LANGMUIR,36(46),14070-14075. |
| MLA | Wang, J,et al."Influence of Krypton Gas Nanobubbles on the Activity of Pepsin".LANGMUIR 36.46(2020):14070-14075. |
入库方式： OAI收割
来源：上海应用物理研究所
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