Label-Free and Three-Dimensional Visualization Reveals the Dynamics of Plasma Membrane-Derived Extracellular Vesicles
文献类型：期刊论文
| 作者 | Wang, KZ; Zhou, LM; Li, J; Liu, WJ; Wei, YH; Guo, Z; Fan, CH; Hu, J; Li, B; Wang, LH |
| 刊名 | NANO LETTERS
 |
| 出版日期 | 2020 |
| 卷号 | 20期号:9页码:6313-6319 |
| 关键词 | EXOSOMES SECRETION CELLS |
| ISSN号 | 1530-6984 |
| DOI | 10.1021/acs.nanolett.0c01735 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Plasma membrane-derived extracellular vesides (PEVs) are carriers of biological molecules that perform special cell cell communications. Nevertheless, the characterization of complicated PEV biology is hampered by the failure of current methods, mainly due to lack of specific labels and insufficient resolution. Here, we employed atomic force microscopy and scanning ion conductance microscopy, both capable of three-dimensional nanoscale resolution, for the label-free visualization of the PEV morphology, release, and uptake at the single-vesicle level. Except for classical microvesicles, we observed a cluster-like PEVs subtype in tumor cells. Moreover, both PEV subtype release times positively correlated with size. Through three-dimensional nanoscale imaging, we visualized the multiform PEV cell interaction behaviors of individual vesicles, which was challenged in conventional PEV imaging. Finally, we developed single-cell manipulation strategies to induce micrometer-sized PEV generation. Collectively, these results revealed the heterogeneous morphology and dynamics of PEVs at the single vesicle level, which provided new insight into the PEV biology. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/32970]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shanghai Adv Res Inst, Shanghai 201210, Peoples R China 2.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Inst Mol Med, Shanghai 200240, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Appl Phys, CAS Key Lab Interfacial Phys & Technol, Shanghai Synchrotron Radiat Facil, Shanghai 201800, Peoples R China 5.Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Sch Chem & Chem Engn, Shanghai 200240, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, KZ,Zhou, LM,Li, J,et al. Label-Free and Three-Dimensional Visualization Reveals the Dynamics of Plasma Membrane-Derived Extracellular Vesicles[J]. NANO LETTERS,2020,20(9):6313-6319. |
| APA | Wang, KZ.,Zhou, LM.,Li, J.,Liu, WJ.,Wei, YH.,...&Wang, LH.(2020).Label-Free and Three-Dimensional Visualization Reveals the Dynamics of Plasma Membrane-Derived Extracellular Vesicles.NANO LETTERS,20(9),6313-6319. |
| MLA | Wang, KZ,et al."Label-Free and Three-Dimensional Visualization Reveals the Dynamics of Plasma Membrane-Derived Extracellular Vesicles".NANO LETTERS 20.9(2020):6313-6319. |
入库方式： OAI收割
来源：上海应用物理研究所
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