Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer
文献类型：期刊论文
| 作者 | Chen, Bin2; Huang, Qiuling4,5; Qu, Zhibei3; Li, Cong3; Li, Qian3; Shi, Jiye4,5; Fan, Chunhai3; Wang, Lihua1,4,5; Zuo, Xiaolei2; Shen, Jianlei3 |
| 刊名 | Angewandte Chemie - International Edition
 |
| 出版日期 | 2020 |
| 关键词 | DNA Conformations Fluorescence Fluorescence microscopy Gene expression Confocal fluorescence microscopy Conformation change Conformation dynamics DNA conformations Drug DNA interactions Fluorescence method G quadruplex structure Single nucleotides |
| ISSN号 | 1433-7851 |
| DOI | 10.1002/anie.202014466 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Variation of DNA conformation is important in regulating gene expression and mediating drug-DNA interactions. However, directly probing transient DNA conformation changes is challenging owing to the dynamic nature of this process. We show a label-free fluorescence method to monitor transient DNA conformation changes in DNA structures with various lengths and shapes using a DNA intercalator, K21. K21 can form transient excimers on the surface of DNA; the ratiometric emission of monomer and excimer correlate to DNA transient conformation stability in numerous DNA structures, including i-motifs, G-quadruplex structures, and single nucleotide mutation at random position. We analyzed the conformation dynamics of a single plasmid before and after enzyme digestion with confocal fluorescence microscopy. This method provides a label-free fluorescence strategy to probe transient conformation changes of DNA structures and has potential in uncovering transient genomic processes in living cells. © 2020 Wiley-VCH GmbH |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/33085]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Bioimaging Center, Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai; 201210, China 2.Institute of Molecular Medicine, Shanghai Key Laboratory for Nucleic Acids Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai; 200127, China; 3.School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai; 200240, China; 4.Division of Physical Biology, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai; 201800, China; 5.University of Chinese Academy of Sciences, Beijing; 100049, China; |
| 推荐引用方式 GB/T 7714 | Chen, Bin,Huang, Qiuling,Qu, Zhibei,et al. Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer[J]. Angewandte Chemie - International Edition,2020. |
| APA | Chen, Bin.,Huang, Qiuling.,Qu, Zhibei.,Li, Cong.,Li, Qian.,...&Li, Jiang.(2020).Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer.Angewandte Chemie - International Edition. |
| MLA | Chen, Bin,et al."Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer".Angewandte Chemie - International Edition (2020). |
入库方式： OAI收割
来源：上海应用物理研究所
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