Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation
文献类型：期刊论文
| 作者 | Wang, Yadi2,3,4; Dai, Wentao1,5; Liu, Zhixiao3; Liu, Jixiang1; Cheng, Jie2,3; Li, Yuanyuan1,5; Li, Xueling6; Hu, Jun2,3; Lü, Junhong2,3 |
| 刊名 | Analytical Chemistry
 |
| 出版日期 | 2021 |
| 卷号 | 93期号:2页码:671-676 |
| ISSN号 | 0003-2700 |
| 关键词 | Stem cells Cell culture Cell proliferation Fourier transform infrared spectroscopy Microoptics Adipogenic differentiations Dynamic heterogeneity FTIR microspectroscopy Human mesenchymal stem cells Infrared micro spectroscopy Mesenchymal stem cell Quantitative evaluation Single cell resolution |
| DOI | 10.1021/acs.analchem.0c04110 |
| 文献子类 | 期刊论文 |
| 英文摘要 | The central relevance of cellular heterogeneity to biological phenomena raises the rational needs for analytical techniques with single-cell resolution. Here, we developed a single-cell FTIR microspectroscopy-based method for the quantitative evaluation of cellular heterogeneity by calculating the cell-to-cell similarity distance of the infrared spectral data. Based on this method, we revealed the infrared phenotypes might reflect the dynamic heterogeneity changes in the cell population during the adipogenic differentiation of the human mesenchymal stem cells. These findings provide an alternative label-free optical approach for quantifying the cellular heterogeneity, and the combination with other single-cell analysis tools will be very helpful for understanding the genotype-to-phenotype relationship in cellular populations. © 2020 American Chemical Society. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/33139]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shanghai Center for Bioinformation Technology, No.1278 Ke Yuan Road, Pudong New District, Shanghai; 201203, China; 2.Shanghai Synchrotron Radiation Facility, Zhangjiang Laboratory, Shanghai Advanced Research Institute, Chinese Academy of Sciences, No. 239 Zhangheng Road, Pudong New District, Shanghai; 201203, China; 3.Shanghai Institute of Applied Physics, Chinese Academy of Sciences, No. 2019 Jia Luo Road, Jiading District, Shanghai; 201800, China; 4.University of Chinese Academy of Sciences, No.19A Yuquan Road, Shijingshan District, Beijing; 100049, China; 5.Shanghai Key Laboratory of Gastric Neoplasms, Shanghai Institute of Digestive Surgery, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, Huangpu District, Shanghai; 200025, China; 6.Shanghai University of Medicine and Health Sciences, National Engineering Research Center for Nanotechnology, No. 28 Jiangchuan East Road, Minhang District, Shanghai; 201318, China |
| 推荐引用方式 GB/T 7714 | Wang, Yadi,Dai, Wentao,Liu, Zhixiao,et al. Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation[J]. Analytical Chemistry,2021,93(2):671-676. |
| APA | Wang, Yadi.,Dai, Wentao.,Liu, Zhixiao.,Liu, Jixiang.,Cheng, Jie.,...&Lü, Junhong.(2021).Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation.Analytical Chemistry,93(2),671-676. |
| MLA | Wang, Yadi,et al."Single-Cell Infrared Microspectroscopy Quantifies Dynamic Heterogeneity of Mesenchymal Stem Cells during Adipogenic Differentiation".Analytical Chemistry 93.2(2021):671-676. |
入库方式： OAI收割
来源：上海应用物理研究所
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