Ultrafast DNA Sensors with DNA Framework-Bridged Hybridization Reactions
文献类型：期刊论文
| 作者 | Li, FQ; Mao, XH; Li, F; Li, M; Shen, JL; Ge, ZL; Fan, CH; Zuo, XL |
| 刊名 | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
 |
| 出版日期 | 2020 |
| 卷号 | 142期号:22页码:9975-9981 |
| 关键词 | NUCLEIC-ACIDS SECONDARY STRUCTURE KINETICS NANOSTRUCTURES ORIGIN |
| ISSN号 | 0002-7863 |
| DOI | 10.1021/jacs.9b13737 |
| 文献子类 | 期刊论文 |
| 英文摘要 | Intracellular DNA-based hybridization reactions generally occur under tension rather than in free states, which are spatiotemporally controlled in physiological conditions. However, how nanomechanical forces affect DNA hybridization efficiencies in in-vitro DNA assays, for example, biosensors or biochips, remains largely elusive. Here, we design DNA framework-based nanomechanical handles that can control the stretching states of DNA molecules. Using a pair of tetrahedral DNA framework (TDF) nanostructured handles, we develop bridge DNA sensors that can capture target DNA with ultrafast speed and high efficiency. We find that the rigid TDF handles bind two ends of a single-stranded DNA (ssDNA) and hold it in a stretched state, with an apparent stretching length comparable to its counterpart of double-stranded DNA (dsDNA) via atomic force microscopy measurement. The DNA stretching effect of ssDNA is then monitored using single-molecule fluorescence energy transfer (FRET), resulting in decreased FRET efficiency in the stretched ssDNA. By controlling the stretching state of ssDNA, we obtained significantly improved hybridization kinetics (within 1 min) and hybridization efficiency (similar to 98%) under the target concentration of 500 nM. The bridge DNA sensors demonstrated high sensitivity (1 fM), high specificity (single mismatch mutation discrimination), and high selectivity (suitable for the detection in serum and blood) under the target concentration of 10 nM. Controlling the stretching state of ssDNA shows great potential in biosensors, bioimaging, and biochips applications. |
| 语种 | 英语 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/33237]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Inst Mol Med, Shanghai Key Lab Nucle Acid Chem & Nanomed,Renji, Shanghai 200127, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Div Phys Biol & Bioimaging Ctr,CAS Key Lab Interf, Shanghai 201800, Peoples R China 3.Shanghai Jiao Tong Univ, Sch Chem & Chem Engn, Shanghai 200127, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, FQ,Mao, XH,Li, F,et al. Ultrafast DNA Sensors with DNA Framework-Bridged Hybridization Reactions[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,2020,142(22):9975-9981. |
| APA | Li, FQ.,Mao, XH.,Li, F.,Li, M.,Shen, JL.,...&Zuo, XL.(2020).Ultrafast DNA Sensors with DNA Framework-Bridged Hybridization Reactions.JOURNAL OF THE AMERICAN CHEMICAL SOCIETY,142(22),9975-9981. |
| MLA | Li, FQ,et al."Ultrafast DNA Sensors with DNA Framework-Bridged Hybridization Reactions".JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 142.22(2020):9975-9981. |
入库方式： OAI收割
来源：上海应用物理研究所
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