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Tunable and Contamination-Free Injection with Microfluidics by Stepinjection

文献类型：期刊论文

作者Hu, Beiyu4,5,6; Ye, Shun3,6; Chen, Dongwei4,6; Xie, Bingliang5,6; Hu, Ran4,6; Qiao, Yuxin4,6; Yu, Yanghuan6; Yu, Haiyan2,6; Zheng, Xu1; Lan, Ying6
刊名ANALYTICAL CHEMISTRY
出版日期2021-10-05
卷号93期号:39页码:13112-13117
ISSN号0003-2700
DOI10.1021/acs.analchem.1c02721
通讯作者Du, Wenbin(wenbin@im.ac.cn)
英文摘要Droplet microfluidics with picoinjection provides significant advantages to multistep reactions and screenings. The Tjunction design for picoinjection is convenient in adding picoliter reagents into passing droplets to initiate reactions. However, conventional picoinjectors face difficulties in eliminating cross-contamination between droplets, preventing them from widespread use in sensitive biological and molecular assays. Here, we introduce stepinjection, which uses a T-junction with a stepped channel design to elevate the diffusional buffer zone into the main channel and consequently increases the pressure difference between droplets and the inlet of the injection channel. To demonstrate the stepinjector's ability to perform contamination-sensitive enzymatic assays, we inject casein fluorescein isothiocyanate (FITC-casein) into a mixture of savinase and savinase-free (labeled with a red fluorescent dye) droplets. We observe no cross-contamination using stepinjection but find a severe cross-talk using an optimal picoinjection design. We envision that the simple, tunable, and reliable stepinjector can be easily integrated in various droplet processing devices, and facilitate various biomedical and biochemical applications including multiplex digital PCR, single-cell sequencing, and enzymatic screening.
WOS关键词HIGH-THROUGHPUT ; DROPLET MICROFLUIDICS
资助项目National Key Research and Development Program of China[2018YFC0310703] ; National Key Research and Development Program of China[2016YFE0205800] ; International Ocean Resource Survey and Exploration[DY135-B-02] ; National Natural Science Foundation of China[21822408] ; National Natural Science Foundation of China[91951103] ; National Natural Science Foundation of China[12072350] ; National Natural Science Foundation of China[11832017] ; National Natural Science Foundation of China[32100073] ; Key Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDB-SSWSMC008] ; Key Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDB-SSW-JSC036] ; CAS Strategic Priority Research Program[XDB22040403] ; Center for Ocean Mega-Science, Chinese Academy of Sciences[KEXUE2019GZ05]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000705944200003
资助机构National Key Research and Development Program of China ; International Ocean Resource Survey and Exploration ; National Natural Science Foundation of China ; Key Program of Frontier Sciences of the Chinese Academy of Sciences ; CAS Strategic Priority Research Program ; Center for Ocean Mega-Science, Chinese Academy of Sciences
源URL[http://dspace.imech.ac.cn/handle/311007/87634]  
专题力学研究所_非线性力学国家重点实验室
通讯作者Du, Wenbin
作者单位1.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China
2.Univ Chinese Acad Sci, Savaid Med Sch, Beijing 100049, Peoples R China
3.Penn State Univ, Biomed Engn Dept, Coll Engn, University Pk, PA 16802 USA
4.Chinese Acad Sci, Inst Microbiol, State Key Lab Transducer Technol, Beijing 100101, Peoples R China
5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
6.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
推荐引用方式
GB/T 7714		 Hu, Beiyu,Ye, Shun,Chen, Dongwei,et al. Tunable and Contamination-Free Injection with Microfluidics by Stepinjection[J]. ANALYTICAL CHEMISTRY,2021,93(39):13112-13117.
APA Hu, Beiyu.,Ye, Shun.,Chen, Dongwei.,Xie, Bingliang.,Hu, Ran.,...&Lan, Y.(2021).Tunable and Contamination-Free Injection with Microfluidics by Stepinjection.ANALYTICAL CHEMISTRY,93(39),13112-13117.
MLA Hu, Beiyu,et al."Tunable and Contamination-Free Injection with Microfluidics by Stepinjection".ANALYTICAL CHEMISTRY 93.39(2021):13112-13117.

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来源：力学研究所

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