The applicability of pH-zone-refining counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example
文献类型：期刊论文
| 作者 | Wang, Qiqi; Chen, Tao; Cui, Yunbin; Li, Si; Jiang, Xinhao; Zhao, Guodong; Li, Yulin ; Zou, Denglang
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2021 |
| 卷号 | 1657 |
| 关键词 | PH-zone-refining counter-current chromatography Biosynthesis Glycosylation Ellagic acid |
| 英文摘要 | Biosynthesis is a research hot-spot in recent years, however, the purification of its final products is a tough work. Liquid stationary phase and large-scale separation ability of PZRCCC could easily avoid the commonly disadvantages occurred in traditional column chromatography. These characteristics makes PZRCCC particularly applicable for final products separation in biosynthesis. In this study, the glycosylation products of ellagic acid by one-pot glycosylation were successfully purified by PZRCCC to show the applicability of PZRCCC for preparative separation of biosynthesis products. An optimized ethyl acetate/n-buthanol/water (3:3:5, v/v/v) system was applied in this study, where 5 mM trifluoroacetic acid (TFA) as the retainer and 30 mM triethylamine (TEA) as the eluter were added. As a result, four ellagic acid glycosylation products, including 51 mg of ellagic acid-4, 3'-O-beta-D-diglucoside (EG-1), 24 mg of ellagic acid-4, 4'-O-beta-D-diglucoside (EG-2), 11 mg of ellagic acid-4-O-beta-D-glucosyl (1 -> 2)-beta-D-glucoside (EG-3) and 64 mg of ellagic acid-4-O-beta-D-glucoside (EG-4) were simultaneously separated from 500 mg of glycosylation crude products, with the purity of 93.3%, 91.2%, 89.4% and 95.5%, respectively. Their structures were identified by spectroscopic analysis. (C) 2021 Elsevier B.V. All rights reserved. |
| 源URL | [http://210.75.249.4/handle/363003/60674]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Qiqi,Chen, Tao,Cui, Yunbin,et al. The applicability of pH-zone-refining counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example[J]. JOURNAL OF CHROMATOGRAPHY A,2021,1657. |
| APA | Wang, Qiqi.,Chen, Tao.,Cui, Yunbin.,Li, Si.,Jiang, Xinhao.,...&Zou, Denglang.(2021).The applicability of pH-zone-refining counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example.JOURNAL OF CHROMATOGRAPHY A,1657. |
| MLA | Wang, Qiqi,et al."The applicability of pH-zone-refining counter-current chromatography for preparative separation of biosynthesis products: Glycosylation products as example".JOURNAL OF CHROMATOGRAPHY A 1657(2021). |
入库方式： OAI收割
来源：西北高原生物研究所
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