Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Chen, Tao; Liu, Yongling; Wang, Shuo; Wei, Yangfei; Song, Zhibo; Yan, Shuping ; Shen, Cheng; Li, Hongmei; Li, Peipei; Li, Yulin
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| 刊名 | SEPARATION SCIENCE AND TECHNOLOGY
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| 出版日期 | 2021 |
| 关键词 | Aconitum pendulum busch first separation counter-current chromatography repeated injection phenolic compounds |
| 英文摘要 | In this study, eight phenolic compounds were obtained from the over-ground parts of the Aconitum pendulum (A. pendulum) Busch through an efficient high-speed counter-current chromatography (HSCCC?method. First, the column chromatography for macroporous resin was used for the treatment of crude extract of over-ground parts of A. pendulum Busch. And then, 30% ethanol elution fraction was transferred to HSCCC for separation using the classic ethyl acetate, n-butanol and water system with the solvent ratio of 7:3:10. To improve parting efficiency, a mode of repeated injection was developed. Two groups of compounds existing in one HPLC peak were effectively separated by HSCCC. The results revealed that HSCCC could effectively isolate compounds with the same or similar polarity. Preparative HPLC was used to perform further purification, and then eight phenolic compounds were separated from the over-ground parts of the A. pendulum Busch for the first time. The current investigation successfully provides a reference for phytochemical research and, comprehensive development and utilization of A. pendulum Busch in the future. Furthermore, HSCCC efficiently separated compounds with similar polarity and its combination with preparative HPLC could play a great role in the separation of natural materials. |
| 源URL | [http://210.75.249.4/handle/363003/60687]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Chen, Tao,Liu, Yongling,Wang, Shuo,et al. Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography[J]. SEPARATION SCIENCE AND TECHNOLOGY,2021. |
| APA | Chen, Tao.,Liu, Yongling.,Wang, Shuo.,Wei, Yangfei.,Song, Zhibo.,...&Li, Yulin.(2021).Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography.SEPARATION SCIENCE AND TECHNOLOGY. |
| MLA | Chen, Tao,et al."Separation of eight phenolic compounds from the over-ground parts of Aconitum pendulum Busch by repeated injection high-speed counter-current chromatography".SEPARATION SCIENCE AND TECHNOLOGY (2021). |
入库方式： OAI收割
来源：西北高原生物研究所
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