Deletion of phosphatidylserine flippase beta-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration
文献类型：期刊论文
| 作者 | Sun, Kuan-Xiang; Jiang, Xiao-Yan; Li, Xiao; Su, Yu-Jing; Wang, Ju-Lin; Zhang, Lin; Yang, Ye-Ming; Zhu, Xian-Jun |
| 刊名 | ZOOLOGICAL RESEARCH
 |
| 出版日期 | 2021 |
| 卷号 | 42期号:5 |
| 关键词 | Tmem30a Skeletal muscle regeneration Knockout mouse model Atp11a Satellite cell |
| 英文摘要 | Phosphatidylserine (PS) is distributed asymmetrically in the plasma membrane of eukaryotic cells. Phosphatidylserine flippase (P4-ATPase) transports PS from the outer leaflet of the lipid bilayer to the inner leaflet of the membrane to maintain PS asymmetry. The beta subunit TMEM30A is indispensable for transport and proper function of P4-ATPase. Previous studies have shown that the ATP11A and TMEM30A complex is the molecular switch for myotube formation. However, the role of Tmem30a in skeletal muscle regeneration remains elusive. In the current study, Tmem30a was highly expressed in the tibialis anterior (TA) muscles of dystrophin-null (mdx) mice and BaCl2-induced muscle injury model mice. We generated a satellite cell (SC)-specific Tmem30a conditional knockout (cKO) mouse model to investigate the role of Tmem30a in skeletal muscle regeneration. The regenerative ability of cKO mice was evaluated by analyzing the number and diameter of regenerated SCs after the TA muscles were injured by BaCl2-injection. Compared to the control mice, the cKO mice showed decreased Pax7(+) and MYH3(+) SCs, indicating diminished SC proliferation, and decreased expression of muscular regulatory factors (MYOD and MYOG), suggesting impaired myoblast proliferation in skeletal muscle regeneration. Taken together, these results demonstrate the essential role of Tmem30a in skeletal muscle regeneration. |
| 源URL | [http://210.75.249.4/handle/363003/60712]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Sun, Kuan-Xiang,Jiang, Xiao-Yan,Li, Xiao,et al. Deletion of phosphatidylserine flippase beta-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration[J]. ZOOLOGICAL RESEARCH,2021,42(5). |
| APA | Sun, Kuan-Xiang.,Jiang, Xiao-Yan.,Li, Xiao.,Su, Yu-Jing.,Wang, Ju-Lin.,...&Zhu, Xian-Jun.(2021).Deletion of phosphatidylserine flippase beta-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration.ZOOLOGICAL RESEARCH,42(5). |
| MLA | Sun, Kuan-Xiang,et al."Deletion of phosphatidylserine flippase beta-subunit Tmem30a in satellite cells leads to delayed skeletal muscle regeneration".ZOOLOGICAL RESEARCH 42.5(2021). |
入库方式： OAI收割
来源：西北高原生物研究所
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