Enrichment and separation of high-polar compounds from Saussurea obvallata using solid-phase extraction combining with offline two-dimensional liquid chromatography
文献类型：期刊论文
| 作者 | Wang, Weidong; Zhu, Yunhe; Jiang, Lei; Mei, Lijuan; Tao, Yanduo; Liu, Zenggen
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
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| 出版日期 | 2021 |
| 卷号 | 44期号:21 |
| 关键词 | enrichment high-polarity compounds offline two-dimensional liquid chromatography Saussurea obvallata solid-phase extraction |
| 英文摘要 | The high-polar compounds from natural products are often used as medicines due to their good bioactivities. However, owing to the complexity and diversity of their structure, the separation of high-polar compounds is still a challenging work. For this, an efficient method for enrichment and separation of the high-polar compounds from Saussurea obvallata was developed. First, the target compounds were enriched from the total extract using a solid-phase extraction method. An offline two-dimensional liquid chromatography method was used for the separation of pure compounds from the enriched sample. After optimization of chromatographic conditions, high separation selectivity of target compounds was obtained on a polar-modified C18 column and a HILIC XAmide column. Hence, a two-dimensional reversed-phase x hydrophilic interaction liquid chromatography system was constructed and enlarged from the analytical level to the preparative level. In the first dimension, four fractions were obtained on the XCharge C18 column with a recovery rate of 71.2%. In the second-dimension preparation on the XAmide column, eight high-polar compounds with more than 96% purity were isolated. All compounds were isolated from Saussurea obvallata for the first time. The results demonstrated that this developed strategy is effective for preparative-scale isolation of high-polar compounds from natural products. |
| 源URL | [http://210.75.249.4/handle/363003/60714]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Weidong,Zhu, Yunhe,Jiang, Lei,et al. Enrichment and separation of high-polar compounds from Saussurea obvallata using solid-phase extraction combining with offline two-dimensional liquid chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2021,44(21). |
| APA | Wang, Weidong,Zhu, Yunhe,Jiang, Lei,Mei, Lijuan,Tao, Yanduo,&Liu, Zenggen.(2021).Enrichment and separation of high-polar compounds from Saussurea obvallata using solid-phase extraction combining with offline two-dimensional liquid chromatography.JOURNAL OF SEPARATION SCIENCE,44(21). |
| MLA | Wang, Weidong,et al."Enrichment and separation of high-polar compounds from Saussurea obvallata using solid-phase extraction combining with offline two-dimensional liquid chromatography".JOURNAL OF SEPARATION SCIENCE 44.21(2021). |
入库方式： OAI收割
来源：西北高原生物研究所
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