A ratiometric fluorescence assay for bleomycin based on dual-emissive chameleon DNA-templated silver nanoclusters
文献类型：期刊论文
| 作者 | Zhu, Shuyun; Liu, Lingyuan; Sun, Jing ; Shi, Fengjin; Zhao, Xian-En
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| 刊名 | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
 |
| 出版日期 | 2021 |
| 卷号 | 252 |
| 关键词 | DNA-templated silver nanoclusters Chameleon Ratiometric nanoprobe Single fluorophore Bleomycin DNA scission |
| 英文摘要 | The authors design dual-emissive DNA-templated silver nanoclusters (DNA-AgNCs) for ratiometric fluorescence sensing bleomycin (BLM) for the first time. A hairpin probe containing two different C-rich DNA templates at two terminals is used to synthesize chameleon DNA-AgNCs, which possess two emission peaks when they are in close proximity. A strong emission is founded at 622 nm (lambda ex = 570 nm) while a weak one is located at 572 nm (lambda ex = 504 nm). Meanwhile, the loop of this probe contains the scission site (5'-GC-3') of BLM. The loop can be cleaved into two parts by BLM-Fe(II) complex, inducing the two DNA-AgNCs away from each other. The fluorescence intensity at 572 nm and 622 nm increases and decreases, respectively. Such chameleon DNA-AgNCs exhibit an obvious fluorescence discoloration from orange to yellow. Therefore, a sensitive ratiometric fluorescent strategy for BLM detection has been proposed with the detection limit of 67 pM. Finally, this ratiometric method is used to detect BLM in serum samples. (C) 2021 Elsevier B.V. All rights reserved. |
| 源URL | [http://210.75.249.4/handle/363003/60808]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Zhu, Shuyun,Liu, Lingyuan,Sun, Jing,et al. A ratiometric fluorescence assay for bleomycin based on dual-emissive chameleon DNA-templated silver nanoclusters[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2021,252. |
| APA | Zhu, Shuyun,Liu, Lingyuan,Sun, Jing,Shi, Fengjin,&Zhao, Xian-En.(2021).A ratiometric fluorescence assay for bleomycin based on dual-emissive chameleon DNA-templated silver nanoclusters.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,252. |
| MLA | Zhu, Shuyun,et al."A ratiometric fluorescence assay for bleomycin based on dual-emissive chameleon DNA-templated silver nanoclusters".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 252(2021). |
入库方式： OAI收割
来源：西北高原生物研究所
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