Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography
文献类型：期刊论文
| 作者 | Dang, Jun; Ma, Jianbin; Du, Yurong; Dawa, YangZom; Wang, Qi; Chen, Chengbiao; Wang, Qilan ; Tao, Yanduo; Ji, Tengfei
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| 出版日期 | 2021 |
| 卷号 | 1170 |
| 关键词 | Saxifraga atrata Polyamide Micro gel chp20p Medium pressure chromatography Bergenin |
| 英文摘要 | In this study, polyamide and MCI GEL? CHP20P were employed as stationary phases in medium pressure chromatography (MPC) for the efficient preparative separation of bergenin from Saxifraga atrata. Ethanol?water, methanol-water, and acetonitrile-water mobile phases all showed good enrichment capacity for bergenin fraction when polyamide was used as a stationary phase. After 5 cycles of polyamide MPC using acetonitrile/water, 1.2 g of bergenin fraction was isolated from 180 g Saxifraga atrata herb. Further purification of this fraction was conducted using MCI GEL? CHP20P styrene-divinylbenzene beads. The bergenin fraction was separated into two fractions, and after three runs of MPC, 714.2 mg of bergenin with purity above 99% was obtained. The results demonstrate that the combination of polyamide and styrene-divinylbenzene MPC can be utilized for preparative isolation of compounds from natural products with high yield and purity. |
| 源URL | [http://210.75.249.4/handle/363003/60815]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Dang, Jun,Ma, Jianbin,Du, Yurong,et al. Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2021,1170. |
| APA | Dang, Jun.,Ma, Jianbin.,Du, Yurong.,Dawa, YangZom.,Wang, Qi.,...&Ji, Tengfei.(2021).Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1170. |
| MLA | Dang, Jun,et al."Large-scale preparative isolation of bergenin standard substance from Saxifraga atrata using polyamide coupled with MCI GEL? CHP20P as stationary phases in medium pressure chromatography".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1170(2021). |
入库方式： OAI收割
来源：西北高原生物研究所
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