A dual-functional colorimetric and fluorescent peptide-based probe for sequential detection of Cu2+ and S2- in 100% aqueous buffered solutions and living cells
文献类型：期刊论文
| 作者 | Wang, Peng; Sun, Liangyu; Wu, Jiang; Yang, Xiupei; Lin, Pengchen; Wang, Min |
| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
 |
| 出版日期 | 2021 |
| 卷号 | 407 |
| 关键词 | Peptide Probe Cu2+ S2- Aqueous medium Cell imaging |
| 英文摘要 | Highly sensitive and selectivite detection of copper ions (Cu2+) and hydrogen sulfide (H2S) have become important research topics due to the potential harmful impacts of these chemicals to human health and the environment. In this study, we report the synthesis of a dual-functional peptide-based probe L (FITC-AhxSer-SerHis), designed to mimic a copper-sulfur metalloprotein, and capable of continuous detection of Cu2+ and S2- based on colorimetric and fluorescent methods. The new probe L displayed excellent turn off fluorescence response and good selectivity for Cu2+ ions via a modification of the tripeptide and fluorescein isothiocyanate group, and produced an obvious color change visible to the naked eye. Furthermore, as an excitable probe, the L-Cu complex could continuously detect S2- with high selectivity and sensitivity in 100% aqueous buffered solutions. The detection limits for fluorescence titration measurements, calculated using the equation 3 sigma/k, were 76.7 nM (Cu2+) and 27.2 nM (S2-), which were well below U.S. EPA safety levels. In addition, L could be cycled to alternately detect Cu2+ and S2-, thereby making it a promising reversible probe. Moreover, L was successfully applied to monitoring Cu2+ and S2- in live RKO cells through fluorescence imaging, exhibiting low cytotoxicity and good cell permeability. |
| 源URL | [http://210.75.249.4/handle/363003/60820]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 推荐引用方式 GB/T 7714 | Wang, Peng,Sun, Liangyu,Wu, Jiang,et al. A dual-functional colorimetric and fluorescent peptide-based probe for sequential detection of Cu2+ and S2- in 100% aqueous buffered solutions and living cells[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,407. |
| APA | Wang, Peng,Sun, Liangyu,Wu, Jiang,Yang, Xiupei,Lin, Pengchen,&Wang, Min.(2021).A dual-functional colorimetric and fluorescent peptide-based probe for sequential detection of Cu2+ and S2- in 100% aqueous buffered solutions and living cells.JOURNAL OF HAZARDOUS MATERIALS,407. |
| MLA | Wang, Peng,et al."A dual-functional colorimetric and fluorescent peptide-based probe for sequential detection of Cu2+ and S2- in 100% aqueous buffered solutions and living cells".JOURNAL OF HAZARDOUS MATERIALS 407(2021). |
入库方式： OAI收割
来源：西北高原生物研究所
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