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Facile engineered polymeric microdevice via co-coupling of phenylboronic acid and Protein A for oriented antibody immobilization enables substantial signal enhancement for an enhanced fluorescence immunoassay

文献类型：期刊论文

作者Chang, Jianguo1,2; Gao, Nailong2; Dai, Peng3; Zhu, Ziming3; You, Hui3; Han, Wei4,5; Li, Lu6,7
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2021-11-01
卷号346
关键词Fluorescence immunoassay AFP Oriented immobilization Micro polymeric device Fluorescence response
DOI10.1016/j.snb.2021.130444
通讯作者You, Hui(usmlhy@iim.ac.cn) ; Han, Wei(hanw@hfcas.ac.cn) ; Li, Lu(deerlyee@hotmail.com)
英文摘要The powerful immunoassay has attracted increasing attention for detecting trace substances. Herein, based on the co-coupling of phenylboronic acid and Protein A for oriented antibodies immobilization, we proposed a polymeric device-based fluorescence immunoassay with enhanced assay performance for Alpha-fetoprotein (AFP) analysis. Unlike traditional physical absorption or chemical covalent conjugation to immobilize antibodies, in this immunoassay, the bioreactivity of AFP antibody was maximumly obtained owing to the oriented immobilization manner. The package density of AFP antibody on the polymethylmethacrylate (PMMA) surface is significantly improved because of the co-coupling case associated distance effect. In addition, the non-specific absorption is greatly inhibited with the combined using of zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) and bovine serum albumin (BSA). These integrated features facilitate the output of strong fluorescence in the presence of AFP but emit very weak background in the absence of AFP. After a typical sandwich immunoreaction, the recorded fluorescence intensity of the immunosensor is proportional to the AFP concentration, showing excellent sensitivity, specificity, and reliability for AFP determination. Comparative studies show the co-coupling is superior than its single coupling counterparts. We envision this unique immunoassay can provide a new avenue for building immunosensors in the fields of disease diagnosis, food safety detection, and environmental monitoring.
WOS关键词BIOSENSORS ; PLATFORM ; BINDING
资助项目Key Research and Development Plan Project of Anhui Province[1704a0802157] ; Key-Area Research and Development Program of Guangdong Province[2019B020219003]
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
出版者ELSEVIER SCIENCE SA
WOS记录号WOS:000691814000003
资助机构Key Research and Development Plan Project of Anhui Province ; Key-Area Research and Development Program of Guangdong Province
源URL[http://ir.hfcas.ac.cn:8080/handle/334002/125231]  
专题中国科学院合肥物质科学研究院
通讯作者You, Hui; Han, Wei; Li, Lu
作者单位1.Chinese Acad Sci, Inst Intelligent Machines, Hefei 230031, Anhui, Peoples R China
2.Univ Sci & Technol China, Hefei 230026, Anhui, Peoples R China
3.Guangxi Univ, Sch Mech Engn, Nanning 530004, Guangxi Provinc, Peoples R China
4.Chinese Acad Sci, Inst Hlth & Med Technol, Hefei Inst Phys Sci, Anhui Prov Key Lab Med Phys & Technol, Hefei 230031, Anhui, Peoples R China
5.Chinese Acad Sci, Hefei Canc Hosp, Hefei 230031, Anhui, Peoples R China
6.Anhui Univ Chinese Med, Sch Integrated Chinese & Western Med, Dept Biochem & Mol Biol, Hefei 230031, Anhui, Peoples R China
7.Anhui Acad Chinese Med, Inst Integrated Chinese & Western Med, Hefei 230031, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Chang, Jianguo,Gao, Nailong,Dai, Peng,et al. Facile engineered polymeric microdevice via co-coupling of phenylboronic acid and Protein A for oriented antibody immobilization enables substantial signal enhancement for an enhanced fluorescence immunoassay[J]. SENSORS AND ACTUATORS B-CHEMICAL,2021,346.
APA Chang, Jianguo.,Gao, Nailong.,Dai, Peng.,Zhu, Ziming.,You, Hui.,...&Li, Lu.(2021).Facile engineered polymeric microdevice via co-coupling of phenylboronic acid and Protein A for oriented antibody immobilization enables substantial signal enhancement for an enhanced fluorescence immunoassay.SENSORS AND ACTUATORS B-CHEMICAL,346.
MLA Chang, Jianguo,et al."Facile engineered polymeric microdevice via co-coupling of phenylboronic acid and Protein A for oriented antibody immobilization enables substantial signal enhancement for an enhanced fluorescence immunoassay".SENSORS AND ACTUATORS B-CHEMICAL 346(2021).

入库方式： OAI收割

来源：合肥物质科学研究院

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