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Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris

文献类型：期刊论文

作者Tian, Miao2,3; Wang, Zhi-Yuan2; Fu, Jun-Ying2; Li, Hui-Wen2; Zhang, Jun2,3; Zhang, Xu-Feng1,3; Luo, Wen2; Lv, Peng-Mei2
刊名PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
出版日期2020-12-30
页码10
ISSN号1082-6068
关键词Box-Behnken design crude glycerol impurities lipase production Pichia pastoris
DOI10.1080/10826068.2020.1870135
通讯作者Luo, Wen(luowen@ms.giec.ac.cn) ; Lv, Peng-Mei(lvpm@ms.giec.ac.cn)
英文摘要Crude glycerol, a by-product of biodiesel production, was employed as the carbon source to produce lipase using Pichia pastoris. Under identical fermentation conditions, cell growth and lipase activity were improved using crude glycerol instead of pure glycerol. The impacts of crude glycerol impurities (methyl ester, grease, glycerol, methanol, and metal ions Na+, Ca2+, and Fe3+) on lipase production were investigated. Impurities accelerated P. pastoris entering the stationary phase. Na+, Ca2+, and grease in waste crude glycerol were the main factors influencing higher lipase activity. Through response surface optimization of Ca2+, Na+, and grease concentrations, lipase activity reached 1437 U/mL (15,977 U/mg), which was 2.5 times that of the control. This study highlights the economical and highly efficient valorization of crude glycerol, demonstrating its possible utilization as a carbon source to produce lipase by P. pastoris without pretreatment.
资助项目China National Key R&D Program during the 13th Five-year Plan Period[2017YFD0601003] ; State Key Program of National Nature Science Foundation of China[51903236] ; State Key Program of National Nature Science Foundation of China[51806225] ; State Key Program of National Nature Science Foundation of China[51606201] ; Guangzhou Science and technology planning project[201804010125] ; Science and technology planning project of Guangdong province[2016A010104008]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
出版者TAYLOR & FRANCIS INC
WOS记录号WOS:000607424900001
资助机构China National Key R&D Program during the 13th Five-year Plan Period ; State Key Program of National Nature Science Foundation of China ; Guangzhou Science and technology planning project ; Science and technology planning project of Guangdong province
源URL[http://ir.giec.ac.cn/handle/344007/32507]  
专题中国科学院广州能源研究所
通讯作者Luo, Wen; Lv, Peng-Mei
作者单位1.Univ Sci & Technol China, Nano Sci & Technol Inst, Suzhou, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Energy Convers, Key Lab Renewable Energy, Guangzhou 510640, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Tian, Miao,Wang, Zhi-Yuan,Fu, Jun-Ying,et al. Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris[J]. PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,2020:10.
APA Tian, Miao.,Wang, Zhi-Yuan.,Fu, Jun-Ying.,Li, Hui-Wen.,Zhang, Jun.,...&Lv, Peng-Mei.(2020).Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris.PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY,10.
MLA Tian, Miao,et al."Crude glycerol impurities improve Rhizomucor miehei lipase production by Pichia pastoris".PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY (2020):10.

入库方式： OAI收割

来源：广州能源研究所

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