Combining Michaelis-Menten theory and enzyme deactivation reactions for the kinetic study of enzymatic hydrolysis by different pretreated sugarcane bagasse
文献类型：期刊论文
| 作者 | Zhang, Yu2 ; Zhang, Ying1,2; Song, Miaojia1,2; Topakas, Evangelos3; Yu, Qiang2; Yuan, Zhenhong2; Wang, Zhongming2; Guo, Ying2
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| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2021-06-01 |
| 卷号 | 105页码:72-78 |
| 关键词 | Enzymatic kinetics Michaelis-Menten theory Enzyme deactivation Cellulase Xylanase |
| ISSN号 | 1359-5113 |
| DOI | 10.1016/j.procbio.2021.03.023 |
| 通讯作者 | Yu, Qiang(yuqiang@ms.giec.ac.cn) |
| 英文摘要 | Conversion of lignocellulose to sugar depends on expensive cellulase and xylanase, hence, improvement of enzyme utilization is important. Enzymatic kinetics can guide researchers toward efficient enzymes utilization. Based on Michaelis-Menten theory and enzyme deactivation reactions, the novel kinetic models describing sugar (glucose and xylose) yield are deduced. The model based on second-order reaction is significantly superior compared to first-order reaction, which is verified by initial and maximum hydrolytic rate. Hydrolytic rate displays a linear relationship with enzyme loading. Sugar yield differs in enzymatic hydrolysis of various pretreated substrates, which is explained by the preferred model. Enzyme deactivation based on the model shows that higher enzyme loading results in reduced activity retention percentage, due to feedback inhibition of products and ineffective adsorption of substrate to enzyme during the hydrolytic process. Therefore, the developed model allows more in-depth study of enzymatic kinetics and provides a useful guide for efficient enzyme utilization. |
| WOS关键词 | LIGNOCELLULOSIC BIOMASS ; CELLULASE DEACTIVATION |
| 资助项目 | National Key Research and Development Project of China[2018YFB1501701] ; National Natural Science Foundation of China[21978288] ; Municipal Science and Technology Project of Guangzhou (China)[201804010081] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:000644228700008 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | National Key Research and Development Project of China ; National Natural Science Foundation of China ; Municipal Science and Technology Project of Guangzhou (China) |
| 源URL | [http://ir.giec.ac.cn/handle/344007/33009]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Yu, Qiang |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.ChInese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China 3.Natl Tech Univ Athens, Sch Chem Engn, Biotechnol Lab, InduBioCat Grp, Athens 15780, Greece |
| 推荐引用方式 GB/T 7714 | Zhang, Yu,Zhang, Ying,Song, Miaojia,et al. Combining Michaelis-Menten theory and enzyme deactivation reactions for the kinetic study of enzymatic hydrolysis by different pretreated sugarcane bagasse[J]. PROCESS BIOCHEMISTRY,2021,105:72-78. |
| APA | Zhang, Yu.,Zhang, Ying.,Song, Miaojia.,Topakas, Evangelos.,Yu, Qiang.,...&Guo, Ying.(2021).Combining Michaelis-Menten theory and enzyme deactivation reactions for the kinetic study of enzymatic hydrolysis by different pretreated sugarcane bagasse.PROCESS BIOCHEMISTRY,105,72-78. |
| MLA | Zhang, Yu,et al."Combining Michaelis-Menten theory and enzyme deactivation reactions for the kinetic study of enzymatic hydrolysis by different pretreated sugarcane bagasse".PROCESS BIOCHEMISTRY 105(2021):72-78. |
入库方式： OAI收割
来源：广州能源研究所
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