A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics
文献类型：期刊论文
| 作者 | Zhang, Jun2,3,4; Luo, Wen3 ; Wang, Zhiyuan3; Chen, Xiaoyan2,3; Lv, Pengmei3; Xu, Jingliang1,3
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| 刊名 | BIORESOURCES AND BIOPROCESSING
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| 出版日期 | 2021-08-19 |
| 卷号 | 8期号:1页码:18 |
| 关键词 | D-Psicose Lipase Escherichia coli Bacillus subtilis Co-culture Metabolomics |
| DOI | 10.1186/s40643-021-00429-8 |
| 通讯作者 | Lv, Pengmei(lvpm@ms.giec.ac.cn) ; Xu, Jingliang(xujl@zzu.edu.cn) |
| 英文摘要 | To develop an economically feasible fermentation process, this study designed a novel bioprocess based on the co-culture of engineered Bacillus subtilis and Escherichia coli for the co-production of extracellular D-psicose and intracellular lipase. After optimizing the co-culture bioprocess, 11.70 g/L of D-psicose along with 16.03 U/mg of lipase was obtained; the glucose and fructose were completely utilized. Hence, the conversion rate of D-psicose reached 69.54%. Compared with mono-culture, lipase activity increased by 58.24%, and D-psicose production increased by 7.08%. In addition, the co-culture bioprocess was explored through metabolomics analysis, which included 168 carboxylic acids and derivatives, 70 organooxygen compounds, 34 diazines, 32 pyridines and derivatives, 30 benzene and substituted derivatives, and other compounds. It also could be found that the relative abundance of differential metabolites in the co-culture system was significantly higher than that in the mono-culture system. Pathway analysis revealed that, tryptophan metabolism and beta-alanine metabolism had the highest correlation and played an important role in the co-culture system; among them, tryptophan metabolism regulates protein synthesis and beta-alanine metabolism, which is related to the formation of metabolic by-products. These results confirm that the co-cultivation of B. subtilis and E. coli can provide a novel idea for D-psicose and lipase biorefinery, and are beneficial for the discovery of valuable secondary metabolites such as turanose and morusin. |
| WOS关键词 | NETWORKS ; GENES |
| 资助项目 | National Key Research and Development Program of China[2019YFB1504003] ; Projects of International Cooperation and Exchanges NSFC[51861145103] ; Natural Science Foundation of Guangdong Province of China[2020A1515010513] ; Guangdong Province Industrial High-tech Sector Science and Technology Project[2017A010105018] ; Municipal Science and Technology Project of Guangzhou[201804010081] ; Guangdong Special Support Program[2017TX04Z109] ; National Natural Science Foundation of China[51606201] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000686785000001 |
| 出版者 | SPRINGER HEIDELBERG |
| 资助机构 | National Key Research and Development Program of China ; Projects of International Cooperation and Exchanges NSFC ; Natural Science Foundation of Guangdong Province of China ; Guangdong Province Industrial High-tech Sector Science and Technology Project ; Municipal Science and Technology Project of Guangzhou ; Guangdong Special Support Program ; National Natural Science Foundation of China |
| 源URL | [http://ir.giec.ac.cn/handle/344007/33651]  |
| 专题 | 中国科学院广州能源研究所 |
| 通讯作者 | Lv, Pengmei; Xu, Jingliang |
| 作者单位 | 1.Zhengzhou Univ, Sch Chem Engn, Zhengzhou 450001, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Guangzhou Inst Energy Convers, Guangdong Prov Key Lab New & Renewable Energy Res, CAS Key Lab Renewable Energy, Guangzhou 510640, Peoples R China 4.Guangdong Ocean Univ, Coll Food Sci & Technol, Guangdong Prov Engn Technol Res Ctr Seafood, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang 524088, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jun,Luo, Wen,Wang, Zhiyuan,et al. A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics[J]. BIORESOURCES AND BIOPROCESSING,2021,8(1):18. |
| APA | Zhang, Jun,Luo, Wen,Wang, Zhiyuan,Chen, Xiaoyan,Lv, Pengmei,&Xu, Jingliang.(2021).A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics.BIORESOURCES AND BIOPROCESSING,8(1),18. |
| MLA | Zhang, Jun,et al."A novel strategy for D-psicose and lipase co-production using a co-culture system of engineered Bacillus subtilis and Escherichia coli and bioprocess analysis using metabolomics".BIORESOURCES AND BIOPROCESSING 8.1(2021):18. |
入库方式： OAI收割
来源：广州能源研究所
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