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Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil

文献类型：期刊论文

作者Zhang, Jun2,3,4; Chen, Huanjun2,4; Wang, Zhiyuan4; Xu, Huijuan4; Luo, Wen4; Xu, Jingliang1,4; Lv, Pengmei4
刊名BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
出版日期2021-09-14
页码9
ISSN号0885-4513
关键词biodiesel Escherichia coli Lipase
DOI10.1002/bab.2247
通讯作者Luo, Wen(luowen@ms.giec.ac.cn) ; Lv, Pengmei(lvpm@ms.giec.ac.cn)
英文摘要Due to its simple, less by-product and environment friendly properties, enzymatic transesterification of oil with short-chain alcohol to biodiesel, fatty acid methyl esters (FAMEs) is considered to be a promising way of green production and has attracted much attention. In this study, FAMEs were synthesized by an enzymatic method with recombinant lipase as catalysts. A thermophilic Bacillus thermocatenulatus lipase 2 (BTL2) was overexpressed in Escherichia coli BL21(DE3) through relative and quantitative analysis using real-time quantitative PCR. The results suggested that the BTL2 gene was overexpressed in E. coli at the mRNA level, and the recombinant strain harboring a high-copy number vectors was selected and applied to fermentation to produce BTL2 with enzyme activity of 35.54 U/mg cells. The recombinant BTL2 solution exhibited excellent resistance to neutral pH, high temperature, and organic solvents after a certain treatment. Finally, the effects of enzymatic transesterification for preparing biodiesel were studied, using rapeseed oil as raw material, as well as BTL2 solution as catalysts, which resulted in 86.04% yield of FAMEs under 50 degrees C for 36 h. The liquid BTL2 was directly used to prepare FAMEs at a higher temperature efficiently, making the thermophilic BTL2 had the potential application value in biodiesel reproduction subsequently.
WOS关键词HIGH-LEVEL EXPRESSION ; THERMOALKALOPHILIC LIPASE
资助项目National Key Research and Development Program of China[2019YFB1504003] ; Projects of International Cooperation and Exchanges NSFC[51861145103] ; Natural Science Foundation of Guangdong Province of China[2020A1515010513] ; Guangdong Special Support Program[2017TX04Z109] ; National Natural Science Foundation of China[51606201]
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology
语种英语
出版者WILEY
WOS记录号WOS:000695671500001
资助机构National Key Research and Development Program of China ; Projects of International Cooperation and Exchanges NSFC ; Natural Science Foundation of Guangdong Province of China ; Guangdong Special Support Program ; National Natural Science Foundation of China
源URL[http://ir.giec.ac.cn/handle/344007/33893]  
专题中国科学院广州能源研究所
通讯作者Luo, Wen; Lv, Pengmei
作者单位1.Zhengzhou Univ, Sch Chem Engn, Zhengzhou, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Guangdong Prov Engn Lab Marine Biol Prod,Guangdon, Coll Food Sci & Technol,Key Lab Adv Proc Aquat Pr, Zhanjiang, Peoples R China
4.Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangdong Prov Key Lab New & Renewable Energy Res, Guangzhou 510640, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Jun,Chen, Huanjun,Wang, Zhiyuan,et al. Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil[J]. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,2021:9.
APA Zhang, Jun.,Chen, Huanjun.,Wang, Zhiyuan.,Xu, Huijuan.,Luo, Wen.,...&Lv, Pengmei.(2021).Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil.BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY,9.
MLA Zhang, Jun,et al."Heat-induced overexpression of the thermophilic lipase from Bacillus thermocatenulatus in Escherichia coli by fermentation and its application in preparation biodiesel using rapeseed oil".BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2021):9.

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来源：广州能源研究所

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