中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Structured illumination microscopy with partially coherent illumination for phase and fluorescent imaging

文献类型:期刊论文

作者Wen, Kai5; Gao, ZhaoLin5; Fang, Xiang5; Liu, Min5; Zheng, JuanJuan3,4,5; Ma, Ying5; Zalevsky, Zeev1,2; Gao, Peng5
刊名OPTICS EXPRESS
出版日期2021-10-11
卷号29期号:21页码:33679-22693
ISSN号1094-4087
DOI10.1364/OE.435783
产权排序3
英文摘要

This study presents a partially coherent illumination based (PCI-based) SIM apparatus for dual-modality (phase and fluorescent) microscopic imaging. The partially coherent illumination (PCI) is generated by placing a rotating diffuser on a monochromatic laser beam, which suppresses speckle noise in the dual-modality images and endows the apparatus with sound sectioning capability. With this system, label-free quantitative phase and super-resolved/sectioned fluorescent images can be obtained for the same sample. We have demonstrated the superiority of the system in phase imaging of transparent cells with high endogenous contrast and in a quantitative manner. In the meantime, we have also demonstrated fluorescent imaging of fluorescent beads, rat tail crosscut, wheat anther, and hibiscus pollen with super-resolution and optical sectioning. We envisage that the proposed method can be applied to many fields, including but not limited to biomedical, industrial, chemistry fields. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

语种英语
出版者OPTICAL SOC AMER
WOS记录号WOS:000708940500078
源URL[http://ir.opt.ac.cn/handle/181661/95150]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
通讯作者Ma, Ying
作者单位1.Bar Ilan Univ, Nano Technol Ctr, Ramat Gan, Israel
2.Bar Ilan Univ, Fac Engn, Ramat Gan, Israel
3.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Peoples R China
4.Xidian Univ, Acad Adv Interdisciplinary Res, Xian 710071, Peoples R China
5.Xidian Univ, Sch Phys & Optoelect Engn, Xian 710071, Peoples R China
推荐引用方式
GB/T 7714
Wen, Kai,Gao, ZhaoLin,Fang, Xiang,et al. Structured illumination microscopy with partially coherent illumination for phase and fluorescent imaging[J]. OPTICS EXPRESS,2021,29(21):33679-22693.
APA Wen, Kai.,Gao, ZhaoLin.,Fang, Xiang.,Liu, Min.,Zheng, JuanJuan.,...&Gao, Peng.(2021).Structured illumination microscopy with partially coherent illumination for phase and fluorescent imaging.OPTICS EXPRESS,29(21),33679-22693.
MLA Wen, Kai,et al."Structured illumination microscopy with partially coherent illumination for phase and fluorescent imaging".OPTICS EXPRESS 29.21(2021):33679-22693.

入库方式: OAI收割

来源:西安光学精密机械研究所

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