Structured illumination microscopy with partially coherent illumination for phase and fluorescent imaging
文献类型:期刊论文
作者 | Wen, Kai5; Gao, ZhaoLin5; Fang, Xiang5; Liu, Min5; Zheng, JuanJuan3,4,5; Ma, Ying5; Zalevsky, Zeev1,2; Gao, Peng5 |
刊名 | OPTICS EXPRESS |
出版日期 | 2021-10-11 |
卷号 | 29期号:21页码:33679-22693 |
ISSN号 | 1094-4087 |
DOI | 10.1364/OE.435783 |
产权排序 | 3 |
英文摘要 | This study presents a partially coherent illumination based (PCI-based) SIM apparatus for dual-modality (phase and fluorescent) microscopic imaging. The partially coherent illumination (PCI) is generated by placing a rotating diffuser on a monochromatic laser beam, which suppresses speckle noise in the dual-modality images and endows the apparatus with sound sectioning capability. With this system, label-free quantitative phase and super-resolved/sectioned fluorescent images can be obtained for the same sample. We have demonstrated the superiority of the system in phase imaging of transparent cells with high endogenous contrast and in a quantitative manner. In the meantime, we have also demonstrated fluorescent imaging of fluorescent beads, rat tail crosscut, wheat anther, and hibiscus pollen with super-resolution and optical sectioning. We envisage that the proposed method can be applied to many fields, including but not limited to biomedical, industrial, chemistry fields. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement |
语种 | 英语 |
出版者 | OPTICAL SOC AMER |
WOS记录号 | WOS:000708940500078 |
源URL | [http://ir.opt.ac.cn/handle/181661/95150] |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
通讯作者 | Ma, Ying |
作者单位 | 1.Bar Ilan Univ, Nano Technol Ctr, Ramat Gan, Israel 2.Bar Ilan Univ, Fac Engn, Ramat Gan, Israel 3.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Peoples R China 4.Xidian Univ, Acad Adv Interdisciplinary Res, Xian 710071, Peoples R China 5.Xidian Univ, Sch Phys & Optoelect Engn, Xian 710071, Peoples R China |
推荐引用方式 GB/T 7714 | Wen, Kai,Gao, ZhaoLin,Fang, Xiang,et al. Structured illumination microscopy with partially coherent illumination for phase and fluorescent imaging[J]. OPTICS EXPRESS,2021,29(21):33679-22693. |
APA | Wen, Kai.,Gao, ZhaoLin.,Fang, Xiang.,Liu, Min.,Zheng, JuanJuan.,...&Gao, Peng.(2021).Structured illumination microscopy with partially coherent illumination for phase and fluorescent imaging.OPTICS EXPRESS,29(21),33679-22693. |
MLA | Wen, Kai,et al."Structured illumination microscopy with partially coherent illumination for phase and fluorescent imaging".OPTICS EXPRESS 29.21(2021):33679-22693. |
入库方式: OAI收割
来源:西安光学精密机械研究所
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。