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Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress

文献类型：期刊论文

作者Zhang, Xia1,5; Zhang, Liangwei3,5; Wang, Xiaoyan2,5; Han, Xiaoyue1,5; Huang, Yan2,5; Li, Bowei3,5; Chen, Lingxin3,4,5
刊名JOURNAL OF HAZARDOUS MATERIALS
出版日期2021-10-05
卷号419页码:10
关键词Fluorescent probe Cysteine Near-infrared imaging Hypoxia reperfusion Environmental stress
ISSN号0304-3894
DOI10.1016/j.jhazmat.2021.126476
通讯作者Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Increasingly grim environmental pollutions are closely related with the occurrence and development of diseases. However, it's obscure how environmental stress disturbs the normal physiological process, and then how endogenous reactive species mend the cases. Hypoxia/reperfusion (H/R), a common and intractable injury in aquaculture and clinic, can induce oxidative stress and ultimately cause irreversible injury to organism. Cysteine (Cys) plays essential roles in maintaining transduction of numerous reactive species and redox homeostasis in subcellular structures, cells and organisms. A great deal of fluorescence research about Cys are focusing on development of selective probes but with poor exploration of the biofunction under environmental stress. Therefore, it is of great significance to examine the bio-effects of Cys against H/R stress. In the present work, we design a fluorescent probe BCy-AC for in situ detecting Cys, the unique Enol-Keto tautomerization of fluorophore BCy-Keto propels the reaction process which will improve the sensitivity and potential application performance of the probe. BCy-AC is conveniently applied to visualize Cys in HT-22 cells, zebrafish and mice tissues. Moreover, imaging results obtained from H/R models reveal that endogenous Cys changes with hypoxia and reperfusion time and Cys pretreatment effectively defend H/R injury in cells and in vivo.
WOS关键词SELECTIVE DETECTION ; GLUTATHIONE DEPLETION ; GOLD NANOPARTICLES ; REACTIVE OXYGEN ; RECENT PROGRESS ; PROBE ; POLYSULFIDES ; LUMINESCENT
资助项目National Natural Science Foundation of China[21976209] ; National Natural Science Foundation of China[21778026] ; National Natural Science Foundation of China[22007005] ; National Natural Science Foundation of China[21804010] ; Science and Technology Innovation Development Plan of Yantai of China[2020MSGY113] ; Shandong Provincial Natural Science Founda-tion Key Project[ZR2020KB022] ; Program of Youth Innovation Promotion Association, CAS[2019217] ; Taishan Scholar Project Special Funding[ts20190962]
WOS研究方向Engineering ; Environmental Sciences & Ecology
语种英语
WOS记录号WOS:000694784800001
资助机构National Natural Science Foundation of China ; Science and Technology Innovation Development Plan of Yantai of China ; Shandong Provincial Natural Science Founda-tion Key Project ; Program of Youth Innovation Promotion Association, CAS ; Taishan Scholar Project Special Funding
源URL[http://ir.yic.ac.cn/handle/133337/29743]  
专题烟台海岸带研究所_近岸生态与环境实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者Chen, Lingxin
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
4.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Shandong Key Lab Coastal Environm Proc, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Xia,Zhang, Liangwei,Wang, Xiaoyan,et al. Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress[J]. JOURNAL OF HAZARDOUS MATERIALS,2021,419:10.
APA Zhang, Xia.,Zhang, Liangwei.,Wang, Xiaoyan.,Han, Xiaoyue.,Huang, Yan.,...&Chen, Lingxin.(2021).Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress.JOURNAL OF HAZARDOUS MATERIALS,419,10.
MLA Zhang, Xia,et al."Visualizing and evaluating mitochondrial cysteine via near-infrared fluorescence imaging in cells, tissues and in vivo under hypoxia/ reperfusion stress".JOURNAL OF HAZARDOUS MATERIALS 419(2021):10.

入库方式： OAI收割

来源：烟台海岸带研究所

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