Flagella is the motile organ of bacteria, and bacteria are driven by the flagella to move toward the best niche that is beneficial to their survival. The rotary direction of flagella is controlled by the flagellar motor. Chemotactic two-component system is an important link between the external signal stimulation and bacterial flagellar movement. The rotor of the flagellar motor is at the output of the bacterial chemotaxis pathway. Both of them contribute to the formation of biofilm, quorum sensing, stress response, and bacterial virulence. Based on their close relationship and important physiological roles and, the advances of the effect of chemotactic two-compo nent system and flagella-related proteins were reviewed in this paper