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Full-Length Transcriptome of Thalassiosira weissflogii as a Reference Resource and Mining of Chitin-Related Genes

文献类型：期刊论文

作者Cheng, Haomiao1,2,3,4; Bowler, Chris5; Xing, Xiaohui6,7,8; Bulone, Vincent6,7,8; Shao, Zhanru1,2,3; Duan, Delin1,2,3,9
刊名MARINE DRUGS
出版日期2021-07-01
卷号19期号:7页码:13
关键词PacBio sequencing full-length transcriptome Thalassiosira weissflogii chitin chitosan
DOI10.3390/md19070392
通讯作者Shao, Zhanru(zrshao@qdio.ac.cn) ; Duan, Delin(dlduan@qdio.ac.cn)
英文摘要beta-Chitin produced by diatoms is expected to have significant economic and ecological value due to its structure, which consists of parallel chains of chitin, its properties and the high abundance of diatoms. Nevertheless, few studies have functionally characterised chitin-related genes in diatoms owing to the lack of omics-based information. In this study, we first compared the chitin content of three representative Thalassiosira species. Cell wall glycosidic linkage analysis and chitin/chitosan staining assays showed that Thalassiosira weissflogii was an appropriate candidate chitin producer. A full-length (FL) transcriptome of T. weissflogii was obtained via PacBio sequencing. In total, the FL transcriptome comprised 23,362 annotated unigenes, 710 long non-coding RNAs (lncRNAs), 363 transcription factors (TFs), 3113 alternative splicing (AS) events and 3295 simple sequence repeats (SSRs). More specifically, 234 genes related to chitin metabolism were identified and the complete biosynthetic pathways of chitin and chitosan were explored. The information presented here will facilitate T. weissflogii molecular research and the exploitation of beta-chitin-derived high-value enzymes and products.
资助项目National Natural Science Foundation of China[41806175] ; Youth Project of Marine Biology and Biotechnology Laboratory in Pilot National Laboratory for Marine Science and Technology[YQ2018NO06]
WOS研究方向Pharmacology & Pharmacy
语种英语
出版者MDPI
WOS记录号WOS:000676813400001
源URL[http://ir.qdio.ac.cn/handle/337002/175748]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Shao, Zhanru; Duan, Delin
作者单位1.Chinese Acad Sci, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Shandong Prov Key Lab Expt Marine Biol, Ctr Ocean Megasci, Inst Oceanol, Qingdao 266071, Peoples R China
3.Pilot Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Univ PSL, Inst Biol ENS IBENS, Dept Biol, Ecole Normale Super,CNRS,INSERM, F-75005 Paris, France
6.Royal Inst Technol KTH, AlbaNova Univ Ctr, Div Glycosci, Dept Chem,Sch Engn Sci Chem Biotechnol & Hlth, S-10691 Stockholm, Sweden
7.Univ Adelaide, Ctr Excellence Plant Cell Walls, Sch Agr Food & Wine, Australian Res Council, Waite Campus, Urrbrae 5064, Australia
8.Univ Adelaide, Sch Agr Food & Wine, Adelaide Glyc, Waite Campus, Urrbrae 5064, Australia
9.Qingdao Bright Moon Seaweed Grp Co Ltd, State Key Lab Bioact Seaweed Subst, Qingdao 266400, Peoples R China
推荐引用方式
GB/T 7714		 Cheng, Haomiao,Bowler, Chris,Xing, Xiaohui,et al. Full-Length Transcriptome of Thalassiosira weissflogii as a Reference Resource and Mining of Chitin-Related Genes[J]. MARINE DRUGS,2021,19(7):13.
APA Cheng, Haomiao,Bowler, Chris,Xing, Xiaohui,Bulone, Vincent,Shao, Zhanru,&Duan, Delin.(2021).Full-Length Transcriptome of Thalassiosira weissflogii as a Reference Resource and Mining of Chitin-Related Genes.MARINE DRUGS,19(7),13.
MLA Cheng, Haomiao,et al."Full-Length Transcriptome of Thalassiosira weissflogii as a Reference Resource and Mining of Chitin-Related Genes".MARINE DRUGS 19.7(2021):13.

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来源：海洋研究所

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