An immunoassay for ochratoxin A using tetramethylrhodamine-labeled ochratoxin A as a probe based on a binding-induced change in fluorescence intensity
文献类型：期刊论文
| 作者 | Li, Yapiao; Zhang, Ning; Wang, Hailin; Zhao, Qiang |
| 刊名 | ANALYST
 |
| 出版日期 | 2020-01-21 |
| 卷号 | 145期号:2页码:651-655 |
| ISSN号 | 0003-2654 |
| 英文摘要 | Ochratoxin A (OTA) is a mycotoxin that can cause health risks to human/animal health. Contamination by OTA can occur in various foods and agricultural products, so sensitive and rapid detection of OTA is crucial. We describe a simple and sensitive fluorescence immunoassay for OTA using tetramethylrhodamine (TMR)-labeled OTA as a fluorescent probe. We conjugated tetramethylrhodamine to OTA through a covalent reaction, and obtained three TMR-OTA isomer probes after purification by high-performance liquid chromatography. All of the fluorescent probes showed high binding affinity to the anti-OTA antibody. Binding of the TMR-OTA probe to the antibody induced strong fluorescence of TMR-OTA due to the possible change in the local environment of TMR caused by affinity binding. In the presence of OTA, the OTA target competitively displaced the bound TMR-OTA probe from the antibody, causing a decrease in fluorescence. Measuring the change in fluorescence enabled rapid detection of OTA. This method was selective and allowed the detection of 1 nM OTA, showing potential for rapid OTA analysis in applications. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44598]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Yapiao,Zhang, Ning,Wang, Hailin,et al. An immunoassay for ochratoxin A using tetramethylrhodamine-labeled ochratoxin A as a probe based on a binding-induced change in fluorescence intensity[J]. ANALYST,2020,145(2):651-655. |
| APA | Li, Yapiao,Zhang, Ning,Wang, Hailin,&Zhao, Qiang.(2020).An immunoassay for ochratoxin A using tetramethylrhodamine-labeled ochratoxin A as a probe based on a binding-induced change in fluorescence intensity.ANALYST,145(2),651-655. |
| MLA | Li, Yapiao,et al."An immunoassay for ochratoxin A using tetramethylrhodamine-labeled ochratoxin A as a probe based on a binding-induced change in fluorescence intensity".ANALYST 145.2(2020):651-655. |
入库方式： OAI收割
来源：生态环境研究中心
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