Chiral Os(II) Polypyridyl Complexes as Enantioselective Nuclear DNA Imaging Agents Especially Suitable for Correlative High-Resolution Light and Electron Microscopy Studies
文献类型：期刊论文
| 作者 | Huang, Rong; Feng, Feng-Ping; Huang, Chun-Hua; Mao, Li; Tang, Miao; Yan, Zhu-Ying; Shao, Bo; Qin, Li; Xu, Tao; Xue, Yan-Hong |
| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2020-01-22 |
| 卷号 | 12期号:3页码:3465-3473 |
| ISSN号 | 1944-8244 |
| 关键词 | osmium(II) polypyridyl complex DNA light-switch ion-pairing enantioselective DNA imaging transmission electron microscopy correlative high-resolution light and electron microscopy |
| 英文摘要 | The high-resolution technique transmission electron microscopy (TEM), with OsO4 as the traditional fixative, is an essential tool for cell biology and medicine. Although OsO(4 )has been extensively used, it is far from perfect because of its high volatility and toxicity. Os(II) polypyridyl complexes like [Os(phen)(2)(dppz)](2+) (phen = 1,10-phenanthroline; dppz = dipyridophenazine) are not only the well-known molecular DNA lightswitches but also the potential ideal candidates for TEM studies. Here, we report that the cell-impermeable cationic [Os(phen)(2)(dppz)](2+) can be preferentially delivered into the live-cell nucleus through ion-pairing with chlorophenolate counter-anions, where it functions as an unparalleled enantioselective nuclear DNA imaging reagent especially suitable for correlative light and electron microscopy (CLEM) studies in both living and fixed cells, which can clearly visualize chromosome aggregation and decondensation during mitosis simultaneously. We propose that the chiral Os(II) polypyridyl complexes can be used as a distinctive group of enantioselective high-resolution CLEM imaging probes for live-cell nuclear DNA studies. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44632]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Huang, Rong,Feng, Feng-Ping,Huang, Chun-Hua,et al. Chiral Os(II) Polypyridyl Complexes as Enantioselective Nuclear DNA Imaging Agents Especially Suitable for Correlative High-Resolution Light and Electron Microscopy Studies[J]. ACS APPLIED MATERIALS & INTERFACES,2020,12(3):3465-3473. |
| APA | Huang, Rong.,Feng, Feng-Ping.,Huang, Chun-Hua.,Mao, Li.,Tang, Miao.,...&Zhu, Ben-Zhan.(2020).Chiral Os(II) Polypyridyl Complexes as Enantioselective Nuclear DNA Imaging Agents Especially Suitable for Correlative High-Resolution Light and Electron Microscopy Studies.ACS APPLIED MATERIALS & INTERFACES,12(3),3465-3473. |
| MLA | Huang, Rong,et al."Chiral Os(II) Polypyridyl Complexes as Enantioselective Nuclear DNA Imaging Agents Especially Suitable for Correlative High-Resolution Light and Electron Microscopy Studies".ACS APPLIED MATERIALS & INTERFACES 12.3(2020):3465-3473. |
入库方式： OAI收割
来源：生态环境研究中心
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