Cytotoxicity of the soluble and insoluble fractions of atmospheric fine particulate matter
文献类型：期刊论文
| 作者 | Liu, Ling; Zhou, Qiuhua; Yang, Xuezhi; Li, Gang; Zhang, Jingzhu; Zhou, Xuehua; Jiang, Wei |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES
 |
| 出版日期 | 2020-05 |
| 卷号 | 91页码:105-116 |
| 关键词 | PM2.5 Soluble fraction Insoluble fraction Membrane rupture Reactive oxygen species |
| ISSN号 | 1001-0742 |
| 英文摘要 | Inhaled atmospheric fine particulate matter (PM2.5) includes soluble and insoluble fractions, and each fraction can interact with cells and cause adverse effects. PM2.5 samples were collected in Jinan, China, and the soluble and insoluble fractions were separated. According to physiochemical characterization, the soluble fraction mainly contains water-soluble ions and organic acids, and the insoluble fraction mainly contains kaolinite, calcium carbonate and some organic carbon. The interaction between PM2.5 and model cell membranes was examined with a quartz crystal microbalance with dissipation (QCM-D) to quantify PM2.5 attachment on membranes and membrane disruption. The cytotoxicity of the total PM2.5 and the soluble and insoluble fractions, was investigated. Negatively charged PM2.5 can adhere to the positively charged membranes and disrupt them. PM2.5 also adheres to negatively charged membranes but does not cause membrane rupture. Therefore, electrostatic repulsion does not prevent PM2.5 attachment, but electrostatic attraction induces remarkable membrane rupture. The human lung epithelial cell line A549 was used for cytotoxicity assessment. The detected membrane leakage, cellular swelling and blebbing indicated a cell necrosis process. Moreover, the insoluble PM2.5 fraction caused a higher cell mortality and more serious cell membrane damage than the soluble fraction. The levels of reactive oxygen species (ROS) enhanced by the two fractions were not significantly different. The findings provide more information to better understand the mechanism of PM2.5 cytotoxicity and the effect of PM2.5 solubility on cytotoxicity. (C) 2020 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44650]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Liu, Ling,Zhou, Qiuhua,Yang, Xuezhi,et al. Cytotoxicity of the soluble and insoluble fractions of atmospheric fine particulate matter[J]. JOURNAL OF ENVIRONMENTAL SCIENCES,2020,91:105-116. |
| APA | Liu, Ling.,Zhou, Qiuhua.,Yang, Xuezhi.,Li, Gang.,Zhang, Jingzhu.,...&Jiang, Wei.(2020).Cytotoxicity of the soluble and insoluble fractions of atmospheric fine particulate matter.JOURNAL OF ENVIRONMENTAL SCIENCES,91,105-116. |
| MLA | Liu, Ling,et al."Cytotoxicity of the soluble and insoluble fractions of atmospheric fine particulate matter".JOURNAL OF ENVIRONMENTAL SCIENCES 91(2020):105-116. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。