Hairpin-Structured Magnetic SERS Sensor for Tetracycline Resistance Gene tetA Detection
文献类型：期刊论文
| 作者 | Lu, Shaoyu; Du, Jingjing; Sun, Zhenli; Jing, Chuanyong |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2020-12-15 |
| 卷号 | 92期号:24页码:16229-16235 |
| ISSN号 | 0003-2700 |
| 英文摘要 | Antibiotic resistance genes (ARGs) have become emerging environmental contaminants, and the effective on-site detection of ARGs is urgently needed. Herein, we constructed a hairpin-structured magnetic sensor for the analysis of a widespread ARG, tetA, using surface-enhanced Raman scattering (SERS). The SERS sensor was assembled by immobilizing core-satellite structured Fe3O4@SiO2-Au with single-stranded DNA in a folded hairpin structure. The SERS sensor exhibited good sensitivity and specificity for the detection of laboratory-synthesized tetA ssDNA fragments. In addition, this SERS strategy is the first of its kind to be employed for monitoring environmental samples in the field, with a limit of detection reaching as low as 25 copies mu L-1. Univariate and multivariate linear regression equations verify the practicability of the SERS sensor for quantitative tetA determination, showing the prospect for an amplification-free alternative platform for sensitive and reliable on-site detection of ARGs in the environment. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44702]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Lu, Shaoyu,Du, Jingjing,Sun, Zhenli,et al. Hairpin-Structured Magnetic SERS Sensor for Tetracycline Resistance Gene tetA Detection[J]. ANALYTICAL CHEMISTRY,2020,92(24):16229-16235. |
| APA | Lu, Shaoyu,Du, Jingjing,Sun, Zhenli,&Jing, Chuanyong.(2020).Hairpin-Structured Magnetic SERS Sensor for Tetracycline Resistance Gene tetA Detection.ANALYTICAL CHEMISTRY,92(24),16229-16235. |
| MLA | Lu, Shaoyu,et al."Hairpin-Structured Magnetic SERS Sensor for Tetracycline Resistance Gene tetA Detection".ANALYTICAL CHEMISTRY 92.24(2020):16229-16235. |
入库方式： OAI收割
来源：生态环境研究中心
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