Intranasal exposure to ZnO nanoparticles induces alterations in cholinergic neurotransmission in rat brain
文献类型：期刊论文
| 作者 | Guo, Zhiling; Zhang, Peng; Luo, Yali; Xie, Heidi Qunhui; Chakraborty, Swaroop; Monikh, Fazel Abdolahpur; Bu, Lijing; Liu, Yiyun; Ma, Yongchao; Zhang, Zhiyong |
| 刊名 | NANO TODAY
 |
| 出版日期 | 2020-12 |
| 卷号 | 35页码:1-8 |
| ISSN号 | 1748-0132 |
| 关键词 | Zinc oxide nanoparticles Brain Cholinergic neurotransmission Acetylcholine Acetylcholinesterase |
| 英文摘要 | The neurotoxicity of inhaled ZnO nanoparticles (NPs) and the underlying mechanisms remain largely unknown. In this study, ZnO NPs (30 +/- 6 nm) were intranasally instilled to rats via a single dose (13 mg Zn/kg BW), with ZnSO4 as the ionic control, and analysis 7-days post exposure. The hippocampus was found to be the main target for Zn accumulation for both ZnO NPs and ZnSO4. Synchrotron radiation based X-ray absorption fine structure (XAFS) analysis showed that no particulate ZnO was found, suggesting the occurrence of dissolution and transformation of ZnO NPs. Multi-omics analysis, including transcriptomics, proteomics and metabolomics, demonstrated that cholinergic neurotransmission was the main biological process affected following both treatments. The release of the key neurotransmitter acetylcholine (ACh) was increased by enhanced ACh synthesis, upregulation of vesicular ACh transporter, and suppression of the activity of ACh hydrolysis enzyme (AChE), either by direct Zn-AChE interaction or a transcriptional down-regulation mechanism. In addition, ZnO NPs and ZnSO4 induced similar molecular consequences and exhibited the same Zn chemical speciation (100 % of Zn complexes) in the hippocampal region evidenced by XAFS analysis, suggesting that the observed biological effects were mainly derived from Zn2+ released from the ZnO NPs. This study not only evidences a new pathway for the impact of ZnO NPs on the brain, but also identifies the origin of the impact as ionic Zn, which provides the basis for safe-by-design of ZnO NPs. (C) 2020 The Author(s). Published by Elsevier Ltd. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44716]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Guo, Zhiling,Zhang, Peng,Luo, Yali,et al. Intranasal exposure to ZnO nanoparticles induces alterations in cholinergic neurotransmission in rat brain[J]. NANO TODAY,2020,35:1-8. |
| APA | Guo, Zhiling.,Zhang, Peng.,Luo, Yali.,Xie, Heidi Qunhui.,Chakraborty, Swaroop.,...&Lynch, Iseult.(2020).Intranasal exposure to ZnO nanoparticles induces alterations in cholinergic neurotransmission in rat brain.NANO TODAY,35,1-8. |
| MLA | Guo, Zhiling,et al."Intranasal exposure to ZnO nanoparticles induces alterations in cholinergic neurotransmission in rat brain".NANO TODAY 35(2020):1-8. |
入库方式： OAI收割
来源：生态环境研究中心
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