Monitoring AuNP Dynamics in the Blood of a Single Mouse Using Single Particle Inductively Coupled Plasma Mass Spectrometry with an Ultralow-Volume High-Efficiency Introduction System
文献类型：期刊论文
| 作者 | Sun, Yuzhen; Liu, Nian ; Wang, Yuanyuan; Yin, Yongguang; Qu, Guangbo; Shi, Jianbo; Song, Maoyong; Hu, Ligang; He, Bin; Liu, Guangliang
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2020-11-17 |
| 卷号 | 92期号:22页码:14872-14877 |
| ISSN号 | 0003-2700 |
| 英文摘要 | Gold nanoparticles (AuNPs) are increasingly being used as diagnostic and therapeutic agents owing to their excellent properties; however, there is not much data available on their dynamics in vivo on a single particle basis in a single mouse. Here, we developed a method for the direct analysis of nanoparticles in trace blood samples based on single particle inductively coupled plasma-mass spectrometry (spICP-MS). A flexible, highly configurable, and precisely controlled sample introduction system was designed by assembling an ultralow-volume autosampler (flow rate in the range of 5-5000 mu L/min) and a customized cyclonic spray chamber (transfer efficiency up to 99%). Upon systematic optimization, the detection limit of the nanoparticle size (LODsize) of AuNPs in ultrapure water was 19 nm, and the detection limit of the nanoparticle number concentration (LODNP) was 8 x 10(4) particle/L. Using a retro-orbital blood sampling method and subsequent dilution, the system was successfully applied to track the dynamic changes in size and concentration for AuNPs in the blood of a single mouse, and the recovery for the blood sample was 111.74%. Furthermore, the concentration of AuNPs in mouse blood reached a peak in a short period of time and, then, gradually decreased. This study provides a promising technique for analyzing and monitoring the size and concentration of nanoparticles in ultralow-volume blood samples with low concentrations, making it a powerful tool for analyzing and understanding the fate of nanoparticles in vivo. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44740]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Sun, Yuzhen,Liu, Nian,Wang, Yuanyuan,et al. Monitoring AuNP Dynamics in the Blood of a Single Mouse Using Single Particle Inductively Coupled Plasma Mass Spectrometry with an Ultralow-Volume High-Efficiency Introduction System[J]. ANALYTICAL CHEMISTRY,2020,92(22):14872-14877. |
| APA | Sun, Yuzhen.,Liu, Nian.,Wang, Yuanyuan.,Yin, Yongguang.,Qu, Guangbo.,...&Jiang, Guibin.(2020).Monitoring AuNP Dynamics in the Blood of a Single Mouse Using Single Particle Inductively Coupled Plasma Mass Spectrometry with an Ultralow-Volume High-Efficiency Introduction System.ANALYTICAL CHEMISTRY,92(22),14872-14877. |
| MLA | Sun, Yuzhen,et al."Monitoring AuNP Dynamics in the Blood of a Single Mouse Using Single Particle Inductively Coupled Plasma Mass Spectrometry with an Ultralow-Volume High-Efficiency Introduction System".ANALYTICAL CHEMISTRY 92.22(2020):14872-14877. |
入库方式： OAI收割
来源：生态环境研究中心
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