Perfluorooctane sulfonate enhances mRNA expression of PPAR gamma and ap2 in human mesenchymal stem cells monitored by long-retained intracellular nanosensor
文献类型：期刊论文
| 作者 | Gao, Yu; Guo, Xixi; Wang, Siyu; Chen, Fubin; Ren, Xiaomin; Xiao, Huaxin; Wang, Lianhui |
| 刊名 | ENVIRONMENTAL POLLUTION
 |
| 出版日期 | 2020-08 |
| 卷号 | 263页码:1-9 |
| 关键词 | Intracellular nanosensor Perfluorooctane sulfonate Real-time function assessment Human mesenchymal stem cells Adipogenic differentiation |
| ISSN号 | 0269-7491 |
| 英文摘要 | Perfluorooctane sulfonate (PFOS) has been widely used as a surface coating for household products. It still exists in living environments despite being restricted, due to its bioaccumulation and long half-life. Studies have shown that PFOS has the ability to induce adipogenic differentiation of human cells. Human mesenchymal stem cells (hMSCs) distributed within the adipose tissue might be a potential target of accumulated PFOS. However, traditional end-point toxicity assays failed to examine the subtle changes of cellular function exposed to low-dose persistent organic pollutants in real time. In the present work, highly sensitive and long-retained (more than 30 days) fluorescence based polymeric nanosensors were developed and employed for real-time assessment of cellular functions. hMSCs were engineered with sensor molecules encapsulated poly (lactic-co-glycolic acid) (PLGA) particles. Once internalized by hMSCs, PLGA particles continuously release and replenish sensor molecules to cytoplasm, resulting in prolonged fluorescence signal against photo bleaching and dilution by exocytosis. With this method, the dynamic changes of viability, ROS induction, and adipogenic differentiation related mRNA expression of hMSCs were monitored. PFOS with the concentration as low as 0.1 mu M can induce cellular ROS and enhance the PPAR gamma and ap2 mRNA expression, suggesting the effect on promoting adipogenic differentiation of hMSCs. (C) 2020 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44770]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Gao, Yu,Guo, Xixi,Wang, Siyu,et al. Perfluorooctane sulfonate enhances mRNA expression of PPAR gamma and ap2 in human mesenchymal stem cells monitored by long-retained intracellular nanosensor[J]. ENVIRONMENTAL POLLUTION,2020,263:1-9. |
| APA | Gao, Yu.,Guo, Xixi.,Wang, Siyu.,Chen, Fubin.,Ren, Xiaomin.,...&Wang, Lianhui.(2020).Perfluorooctane sulfonate enhances mRNA expression of PPAR gamma and ap2 in human mesenchymal stem cells monitored by long-retained intracellular nanosensor.ENVIRONMENTAL POLLUTION,263,1-9. |
| MLA | Gao, Yu,et al."Perfluorooctane sulfonate enhances mRNA expression of PPAR gamma and ap2 in human mesenchymal stem cells monitored by long-retained intracellular nanosensor".ENVIRONMENTAL POLLUTION 263(2020):1-9. |
入库方式： OAI收割
来源：生态环境研究中心
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