Protonation-Suppression-Free LC-MS/MS Analysis for Profiling of DNA Cytosine Modifications in Adult Mice
文献类型：期刊论文
| 作者 | Mo, Jiezhen; Liang, Ziyu; Lu, Meiling; Wang, Hailin |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2020-06-02 |
| 卷号 | 92期号:11页码:7430-7436 |
| ISSN号 | 0003-2700 |
| 英文摘要 | DNA cytosine modifications are important epigenetic marks. To elucidate their roles by a large scale of comparative studies, it is important to quantify the abundance of DNA cytosine modifications accurately. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a golden option. The performance of LC-MS/MS is heavily dependent on the ionization or protonation of target analytes. Initially, we found that two factors, DNA hydrolysate buffer and residual coeluted nucleosides, might greatly suppress the protonation of 5-(hydroxymethyl)-2'deoxycytidine (5hmdC). Surprisingly, ammonium bicarbonate can eliminate the suppression caused by both factors. Mechanistically, ammonium bicarbonate increases the protonation capacity in the gas phase and facilitates proton transfer to the target nucleosides. Benefiting from these findings, we developed a suppression-free, sensitive, and robust ultrahigh-performance LC-MS/MS assay for massive detection of three DNA cytosine modifications, including 5-methyl-2'-deoxycytidine (5mdC), 5hmdC, and 5-formyl-2'-deoxycytidine (5fdC). In 30 consecutive analyses, the relative standard deviation (RSD) of the 5hmdC and 5fdC peak areas is 2.0% and 3.2%, respectively. In this case, no stable isotope-labeled standard is required for internal calibration. We further performed a comprehensive profiling of DNA cytosine modifications in 26 tissues of age-different C57BL/6N mice. Interestingly, we found that only liver 5hmdC abundance increases with the increasing age of adult mice, suggesting that liver 5hmdC might be a potential indicator of age in adulthood. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44788]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Mo, Jiezhen,Liang, Ziyu,Lu, Meiling,et al. Protonation-Suppression-Free LC-MS/MS Analysis for Profiling of DNA Cytosine Modifications in Adult Mice[J]. ANALYTICAL CHEMISTRY,2020,92(11):7430-7436. |
| APA | Mo, Jiezhen,Liang, Ziyu,Lu, Meiling,&Wang, Hailin.(2020).Protonation-Suppression-Free LC-MS/MS Analysis for Profiling of DNA Cytosine Modifications in Adult Mice.ANALYTICAL CHEMISTRY,92(11),7430-7436. |
| MLA | Mo, Jiezhen,et al."Protonation-Suppression-Free LC-MS/MS Analysis for Profiling of DNA Cytosine Modifications in Adult Mice".ANALYTICAL CHEMISTRY 92.11(2020):7430-7436. |
入库方式： OAI收割
来源：生态环境研究中心
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