Silver nanoparticles (AgNPs) and AgNO3 perturb the speci fication of human hepatocyte-like cells and cardiomyocytes
文献类型：期刊论文
| 作者 | Hu, Bowen; Yin, Nuoya; Yang, Renjun; Liang, Shengxian; Liang, Shaojun; Faiola, Francesco |
| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2020-07-10 |
| 卷号 | 725页码:1-10 |
| ISSN号 | 0048-9697 |
| 关键词 | Human embryonic stem cells (hESCs) Silver nanoparticles (AgNPs) Developmental toxicity Hepatocyte-like cells Cardiomyocytes Stem cell toxicology |
| 英文摘要 | Silver nanoparticles (AgNPs) are commonly utilized industrial compounds mostly because of their antimicrobial properties. Nevertheless, our understanding of their potential developmental toxicity in humans is still limited. Embryonic stem cells (ESCs) are powerful in vitro tools for developmental toxicity assessments of chemicals. Here, we evaluated the potential developmental toxicity during early embryogenesis of AgNPs and AgNO3 with human ESC (hESC)-based differentiation systems in vitro. We found that human relevant concentrations of AgNPs and Ag ions affected the specification of two of the three primary germ layers, endoderm and mesoderm, without drastically affecting ectoderm. Furthermore, the two forms of Ag impaired the generation and functions of hepatocytes-like cells derived from endoderm, by decreasing the expression of important liver markers such as AFP, ALB, and HNF4A, and altering glycogen storage. When considering cardiac development, AgNPs and AgNO3 manifested opposite adverse effects, in that AgNPs increased while AgNO3 decreased the expression of typical cardiac markers (NKX2.5, MYH6, and ISL) in hESC-derived cardiomyocytes. In conclusion, our findings argue for a potential developmental toxicity of AgNP doses we are exposed to, or levels detected in the human body, especially at very early stages during embryogenesis, and which may not be just due to Ag leakage. Moreover, mesendoderm-derived cell types, tissues and organs may be more prone to AgNP toxicity than ectoderm lineages. (C) 2020 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44807]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Hu, Bowen,Yin, Nuoya,Yang, Renjun,et al. Silver nanoparticles (AgNPs) and AgNO3 perturb the speci fication of human hepatocyte-like cells and cardiomyocytes[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,725:1-10. |
| APA | Hu, Bowen,Yin, Nuoya,Yang, Renjun,Liang, Shengxian,Liang, Shaojun,&Faiola, Francesco.(2020).Silver nanoparticles (AgNPs) and AgNO3 perturb the speci fication of human hepatocyte-like cells and cardiomyocytes.SCIENCE OF THE TOTAL ENVIRONMENT,725,1-10. |
| MLA | Hu, Bowen,et al."Silver nanoparticles (AgNPs) and AgNO3 perturb the speci fication of human hepatocyte-like cells and cardiomyocytes".SCIENCE OF THE TOTAL ENVIRONMENT 725(2020):1-10. |
入库方式： OAI收割
来源：生态环境研究中心
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