Transcriptomic analysis identifies early cellular and molecular events by which estrogen disrupts testis differentiation and causes feminization in Xenopus laevis
文献类型：期刊论文
| 作者 | Li, Yuanyuan; Shen, Yanping; Li, Jinbo ; Cai, Man; Qin, Zhanfen
|
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2020-09 |
| 卷号 | 226页码:1-9 |
| 关键词 | Estrogenic endocrine-disrupting chemicals Xenopus laevis Testis differentiation Feminization |
| ISSN号 | 0166-445X |
| 英文摘要 | Extensive studies have shown that estrogenic endocrine-disrupting chemicals (EDCs) can disrupt testis differentiation and even cause feminization in vertebrates. However, little is known about the mechanisms by which estrogenic EDCs disrupt testis differentiation. Here, we employed Xenopus laevis, a model amphibian species sensitive to estrogenic EDCs, to explore the molecular and cellular events by which 17 beta-estradiol (E2) disrupts testis differentiation and causes feminization. Following waterborne exposure to E2 from stage 45/46, genetically male X. laevis were confirmed to undergo testis differentiation inhibition and ovary differentiation activation at stages 52 and 53, ultimately displaying gonadal feminization at stage 66. Using a time-course RNA sequencing approach, we then identified thousands of differentially expressed transcripts (DETs) in genetically male gonad-mesonephros complexes at stages 48, 50 and 52 (the window for testis differentiation) between E2 treatment and the control. Enrichment analysis suggests alterations in cell proliferation, extracellular matrix, and cell motility following E2 exposure. Further verification by multiple methods demonstrated that E2 inhibited cell proliferation, disrupted extracellular matrix, and altered cell motility in the genetically male gonads compared with controls, implying that these events together contributed to testis differentiation disruptions and feminization in X. laevis. This study for the first time uncovered some of the early molecular and cellular events by which estrogen disrupts testicular differentiation and causes feminization in X. laevis. These new findings improve our understanding of the mechanisms by which estrogenic EDCs disrupt testicular differentiation in vertebrates. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44835]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, Yuanyuan,Shen, Yanping,Li, Jinbo,et al. Transcriptomic analysis identifies early cellular and molecular events by which estrogen disrupts testis differentiation and causes feminization in Xenopus laevis[J]. AQUATIC TOXICOLOGY,2020,226:1-9. |
| APA | Li, Yuanyuan,Shen, Yanping,Li, Jinbo,Cai, Man,&Qin, Zhanfen.(2020).Transcriptomic analysis identifies early cellular and molecular events by which estrogen disrupts testis differentiation and causes feminization in Xenopus laevis.AQUATIC TOXICOLOGY,226,1-9. |
| MLA | Li, Yuanyuan,et al."Transcriptomic analysis identifies early cellular and molecular events by which estrogen disrupts testis differentiation and causes feminization in Xenopus laevis".AQUATIC TOXICOLOGY 226(2020):1-9. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。