Arsenic transformation mediated by gut microbiota affects the fecundity of Caenorhabditis elegans
文献类型：期刊论文
| 作者 | Zhou, Guo-Wei; Yang, Xiao-Ru; Zheng, Fei; Zhang, Zi-Xing; Zheng, Bang-Xiao; Zhu, Yong-Guan; Xue, Xi-Mei |
| 刊名 | ENVIRONMENTAL POLLUTION
 |
| 出版日期 | 2020-05 |
| 卷号 | 260页码:1-7 |
| 关键词 | Arsenic biotransformation Gut microbe C. elegans Fecundity |
| ISSN号 | 0269-7491 |
| 英文摘要 | Arsenic biotransformation has been discovered in guts of soil invertebrates. Reproduction of invertebrates is sensitive to arsenic contamination in soils. However, little is known about the impact of gut microbe-mediated arsenic biotransformation on the fecundity of invertebrates. Here, Caenorhabditis elegans was firstly pre-fed with Escherichia Coll BL21 possessing the capability of reducing arsenate [As(V)] or BL21M having the ability to reduce As(V) and methylate arsenite [As(III)], then inoculated worms were transferred to inactive E. coll. AW3110 (harboring no arsenic transformation gene)-seeded plates treated with As(V) at different concentrations. Quantification of gut microbes showed that both E. coli. BL21 and BL21M stably colonized in the guts after worms were cultured on inactive E. coli. AW3110-seeded plates for 72 h. The analysis of arsenic species indicated that there was As(III) in C. elegans guts colonized with E. coli. BL21, As(III) and dimethylarsinic acid [DMAs(V)] in C. elegans guts with E. coli. BL21M exposed to As(V) for 6 h. After treatment of 100 mu M As(V), decrease in brood sizes was observed for worms that were colonized with E. coll. BL21 or BL21M compared to that with AW3110 in the guts. The levels of vitellogenin (VTG), glutathione S-transferases (GST) and superoxide dismutase (SOD), closely linked to reproduction and antioxidation-linked indicators, were the highest in worms whose guts colonized with E. coli. BL21, followed by worms colonized with E. coli. BL21M and worms colonized with inactive E. coli. AW3110 exposed to As(V). Our results indicated the toxic impact of As(III) and DMAs(V) produced by gut microbes on reproduction of C. elegans. The work provides novel insight into the interplay between arsenic biotransformation mediated by gut microbes and the host fecundity in soils. (C) 2020 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44220]  |
| 专题 | 生态环境研究中心_城市与区域生态国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhou, Guo-Wei,Yang, Xiao-Ru,Zheng, Fei,et al. Arsenic transformation mediated by gut microbiota affects the fecundity of Caenorhabditis elegans[J]. ENVIRONMENTAL POLLUTION,2020,260:1-7. |
| APA | Zhou, Guo-Wei.,Yang, Xiao-Ru.,Zheng, Fei.,Zhang, Zi-Xing.,Zheng, Bang-Xiao.,...&Xue, Xi-Mei.(2020).Arsenic transformation mediated by gut microbiota affects the fecundity of Caenorhabditis elegans.ENVIRONMENTAL POLLUTION,260,1-7. |
| MLA | Zhou, Guo-Wei,et al."Arsenic transformation mediated by gut microbiota affects the fecundity of Caenorhabditis elegans".ENVIRONMENTAL POLLUTION 260(2020):1-7. |
入库方式： OAI收割
来源：生态环境研究中心
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