Insights into palladium nanoparticles produced by Shewanella oneidensis MR-1: Roles of NADH dehydrogenases and hydrogenases
文献类型：期刊论文
| 作者 | Yang, Zhen-Ni; Hou, Ya-Nan; Zhang, Bo; Cheng, Hao-Yi ; Yong, Yang-Chun; Liu, Wen-Zong; Han, Jing-Long; Liu, Shuang-Jiang; Wang, Ai-Jie
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| 刊名 | ENVIRONMENTAL RESEARCH
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| 出版日期 | 2020-12 |
| 卷号 | 191页码:1-11 |
| 关键词 | Shewanella oneidensis NADH dehydrogenase Hydrogenase Palladium nanoparticle Cytochromes |
| ISSN号 | 0013-9351 |
| 英文摘要 | Biologically synthesized palladium nanoparticles (bio-Pd) have attracted considerable interest as promising green catalysts for environmental remediation. However, the mechanisms by which microorganisms produce bioPd remain unclear. In the present study, we investigated the roles of Shewanella oneidensis MR-1 and its NADH dehydrogenases and hydrogenases (HydA and HyaB) in bio-Pd production using formate as the electron donor. The roles of NADH dehydrogenases and hydrogenases were studied by inhibiting NADH dehydrogenases and using hydrogenase mutants (Delta hydA, Delta hyaB, and Delta hydA Delta hyaB), respectively. The results showed similar to 97% reduction of palladium by S. oneidensis MR-1 after 24 h using 250 mu M palladium and 500 mu M formate. Electron microscopy images showed the presence of bio-Pd on both the outer and cytoplasmic membranes of S. oneidensis MR-1. However, the inhibition of NADH dehydrogenases in S. oneidensis MR-1 resulted in only similar to 61% reduction of palladium after 24 h, and bio-Pd were not found on the outer membrane. The mutants lacking one or two hydrogenases removed 91-96% of palladium ions after 24 h and showed more cytoplasmic bio-Pd but less periplasmic bio-Pd. To the best of our knowledge, this is the first study to demonstrate the role of NADH dehydrogenases of S. oneidensis MR-1 in the formation of bio-Pd on the outer membrane. It also demonstrates that the hydrogenases (especially HyaB) of S. oneidensis MR-1 contribute to the formation of bio-Pd in the periplasmic space. This study provides mechanistic insights into the production of biogenic metal nanoparticles towards their possible use in industrial and environmental applications. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45175]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Yang, Zhen-Ni,Hou, Ya-Nan,Zhang, Bo,et al. Insights into palladium nanoparticles produced by Shewanella oneidensis MR-1: Roles of NADH dehydrogenases and hydrogenases[J]. ENVIRONMENTAL RESEARCH,2020,191:1-11. |
| APA | Yang, Zhen-Ni.,Hou, Ya-Nan.,Zhang, Bo.,Cheng, Hao-Yi.,Yong, Yang-Chun.,...&Wang, Ai-Jie.(2020).Insights into palladium nanoparticles produced by Shewanella oneidensis MR-1: Roles of NADH dehydrogenases and hydrogenases.ENVIRONMENTAL RESEARCH,191,1-11. |
| MLA | Yang, Zhen-Ni,et al."Insights into palladium nanoparticles produced by Shewanella oneidensis MR-1: Roles of NADH dehydrogenases and hydrogenases".ENVIRONMENTAL RESEARCH 191(2020):1-11. |
入库方式： OAI收割
来源：生态环境研究中心
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