Why do DIETers like drinking: Metagenomic analysis for methane and energy metabolism during anaerobic digestion with ethanol
文献类型：期刊论文
| 作者 | Zhao, Zhiqiang; Wang, Jianfeng; Li, Yang; Zhu, Tingting ; Yu, Qilin; Wang, Tingting; Liang, Song; Zhang, Yaobin
|
| 刊名 | WATER RESEARCH
 |
| 出版日期 | 2020-03-15 |
| 卷号 | 171页码:1-13 |
| 关键词 | Anaerobic digestion Direct interspecies electron transfer (DIET) Metagenomic analysis Methanothrix species Ethanol |
| ISSN号 | 0043-1354 |
| 英文摘要 | Stimulating Methanothrix-dominant communities with ethanol is recently considered as a promising strategy of improving the efficiency and stability of anaerobic digestion (AD), while the effects on methanogenic pathway and energy metabolism linked to the establishment of direct interspecies electron transfer (DIET) were not investigated yet. The results showed that, Methanothrix species were the dominant and metabolically active methanogens in the methanogenic sludge fed with the ethanol-type fermentation products, and the abundance of genes that encoded the key enzymes involved in the reduction of carbon dioxide was significantly higher than that fed with the other products, such as propionate and butyrate. Conversely, the abundance of genes that encoded the key enzymes involved in acetate decarboxylation among all the methanogenic sludge were nearly same. In the presence of ethanol, the abundance of gene for pilA significantly increased. The gene for pliA was primarily derived from Sphaerochaeta, Sedimentibacter and Pseudomonas species that were specially abundant and metabolically active. Further analysis showed that, the abundance of genes that encoded WA-type ATPase in the methanogenic digesters fed with the ethanol-type fermentation products was 1.3-1.5 folds higher than that fed with the other products. As a result, the concentration of total ATP in the cells was increased by 1.8-2.3 folds. These results, and the fact that DIET is the only electron donor to support the reduction of carbon dioxide in Methanothrix species for the first time revealed the mechanisms involved in the establishment of DIET-based methanogenic metabolism with ethanol. (C) 2019 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45223]  |
| 专题 | 生态环境研究中心_中国科学院环境生物技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhao, Zhiqiang,Wang, Jianfeng,Li, Yang,et al. Why do DIETers like drinking: Metagenomic analysis for methane and energy metabolism during anaerobic digestion with ethanol[J]. WATER RESEARCH,2020,171:1-13. |
| APA | Zhao, Zhiqiang.,Wang, Jianfeng.,Li, Yang.,Zhu, Tingting.,Yu, Qilin.,...&Zhang, Yaobin.(2020).Why do DIETers like drinking: Metagenomic analysis for methane and energy metabolism during anaerobic digestion with ethanol.WATER RESEARCH,171,1-13. |
| MLA | Zhao, Zhiqiang,et al."Why do DIETers like drinking: Metagenomic analysis for methane and energy metabolism during anaerobic digestion with ethanol".WATER RESEARCH 171(2020):1-13. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。