High-Throughput Single Cell Analysis Reveals the Heterogeneity of QDots-Induced Response in Macrophages
文献类型：期刊论文
| 作者 | Wang, Yuanyuan; Hu, Ligang; Xia, Tian; Yao, Xinglei; Yin, Yongguang; Wang, Fengbang; Song, Maoyong ; Jiang, Guibin
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| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS
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| 出版日期 | 2020-05-12 |
| 卷号 | 7期号:5页码:337-342 |
| ISSN号 | 2328-8930 |
| 英文摘要 | The ability to assess the responses of cells exposed to nanoparticles at the single cell level will lead to mechanistic understanding of their toxicity and safety. Here, we demonstrated the heterogeneity of cell uptake and cellular responses in a QDots-exposed macrophage using a high-content screening (HCS) analysis. The dynamic uptake and intracellular location of QDots were characterized by HCS due to its fluorescence. We further verified the association between fluorescence intensity and mass concentration of intracellular QDots using combined methods. The heterogeneity of multiple cell responses, including apoptosis, autophagy, calcium influx, mitochondria membrane potential, nucleus size, and reactive oxygen species generation, was analyzed for fingerprinting of the cytotoxicity of QDots at the single cell level. On the basis of the heterogeneity and the established dose-response relationship, we illustrated that ROS generation was the most sensitive parameter to intracellular QDots. This study makes an important methodological contribution to nanotoxicology with much higher throughput and accuracy at the single cell level. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44875]  |
| 专题 | 生态环境研究中心_环境纳米材料实验室 |
| 推荐引用方式 GB/T 7714 | Wang, Yuanyuan,Hu, Ligang,Xia, Tian,et al. High-Throughput Single Cell Analysis Reveals the Heterogeneity of QDots-Induced Response in Macrophages[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS,2020,7(5):337-342. |
| APA | Wang, Yuanyuan.,Hu, Ligang.,Xia, Tian.,Yao, Xinglei.,Yin, Yongguang.,...&Jiang, Guibin.(2020).High-Throughput Single Cell Analysis Reveals the Heterogeneity of QDots-Induced Response in Macrophages.ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS,7(5),337-342. |
| MLA | Wang, Yuanyuan,et al."High-Throughput Single Cell Analysis Reveals the Heterogeneity of QDots-Induced Response in Macrophages".ENVIRONMENTAL SCIENCE & TECHNOLOGY LETTERS 7.5(2020):337-342. |
入库方式： OAI收割
来源：生态环境研究中心
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