In vitro estrogen-disrupting effects of organophosphate flame retardants
文献类型：期刊论文
| 作者 | Ji, Xiaoya; Li, Na ; Ma, Mei; Rao, Kaifeng; Wang, Zijian
|
| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2020-07-20 |
| 卷号 | 727页码:1-10 |
| ISSN号 | 0048-9697 |
| 关键词 | OPFRs Estrogen-disrupting effects ER alpha Yeast two-hybrid assay MVLN cell assay E-SCREEN assay |
| 英文摘要 | Organophosphate flame retardants (OPFRs), as substitutes for polybrominated diphenyl ethers (PBDEs), are frequently detected in the environment and biota due to their widespread use. Thus, there is a need to investigate their potential estrogen-disrupting effects and possible mechanisms of action in an effort to obtain a better risk assessment. In this study, we characterized the activities on estrogen receptor a (ER alpha) and the estrogendisrupting potential of fourteen OPFRs, TMP, TEP, TPP, TnBP, TiBP, THP, TPhP, TCP, DPK, MDPP, IDPP, CDP, IPPDP and MPhP, using three in vitro assays representing different specific modes of action (MoAs). In the yeast two-hybrid assay, no OPFRs induced agonistic activity, but TiBP, DPK, TPhP, MDPP, CDP and IPPDP were shown to be hydrophobicity-dependent antagonists and to compete with E2 for binding to ER alpha. In the MVLN cell assay, TPhP was the only OPFR among the 14 tested that was able to activate ER alpha-estrogen responsive element (ERE) pathways. The results fromthe E-SCREEN assay showed that all tested OPFRs except TMP had estrogenic properties, and G protein-coupled receptor 30 (GPR30) was involved in the estrogenicity of eight OPFRs, TiBP, THP, TPhP, TCP, MDPP, IPPDP, CDP andMPhP. It was also found that in the E-SCREEN assay, the estrogenicity of alkyl-OPFRs but not aryl-OPFRs was closely correlated to hydrophobicity. Our research suggested that most OPFRs were estrogen disruptors, but their related mechanisms were complex and might involve ER alpha-mediated and/or ER alpha-independent pathways. Further in vitro studies concerning the estrogenic effects and involved mechanisms of OPFRs, aswell as comprehensive evaluations of OPFRs including health and ecological assessments are needed to determine whether they are safe substitutes for PBDEs. (C) 2020 Published by Elsevier B.V. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45310]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Ji, Xiaoya,Li, Na,Ma, Mei,et al. In vitro estrogen-disrupting effects of organophosphate flame retardants[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,727:1-10. |
| APA | Ji, Xiaoya,Li, Na,Ma, Mei,Rao, Kaifeng,&Wang, Zijian.(2020).In vitro estrogen-disrupting effects of organophosphate flame retardants.SCIENCE OF THE TOTAL ENVIRONMENT,727,1-10. |
| MLA | Ji, Xiaoya,et al."In vitro estrogen-disrupting effects of organophosphate flame retardants".SCIENCE OF THE TOTAL ENVIRONMENT 727(2020):1-10. |
入库方式： OAI收割
来源：生态环境研究中心
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