In vitro oxidative stress, mitochondrial impairment and G1 phase cell cycle arrest induced by alkyl-phosphorus-containing flame retardants
文献类型：期刊论文
| 作者 | Yuan, Shengwu; Zhu, Kongrui; Ma, Mei; Zhu, Xiaoshan; Rao, Kaifeng; Wang, Zijian |
| 刊名 | CHEMOSPHERE
 |
| 出版日期 | 2020-06 |
| 卷号 | 248页码:1-9 |
| 关键词 | Phosphorus-containing flame retardants Oxidative stress DNA damage Mitochondrial impairment Cell apoptosis |
| ISSN号 | 0045-6535 |
| 英文摘要 | Phosphorus-containing flame retardants (PFRs) have been frequently detected in various environmental samples at relatively high concentrations and are considered emerging environmental pollutants. However, their biological effects and the underlying mechanism remain unclear, especially alkyl-PFRs. In this study, a battery of in vitro bioassays was conducted to analyze the cytotoxicity, oxidative stress, mitochondrial impairment, DNA damage and the involved molecular mechanisms of several selected alkyl-PFRs. Results showed that alkyl-PFRs induced structural related toxicity, where alkyl-PFRs with higher logKow values induced higher cytotoxicity. Long-chain alkyl-PFRs caused mitochondrial and DNA damage, resulting from intracellular reactive oxygen species (ROS) and mitochondrial superoxide overproduction; while short-chain alkyl-PFRs displayed adverse outcomes by significantly impairing mitochondria without obvious ROS generation. In addition, alkyl-PFRs caused DNA damage-induced cell cycle arrest, as determined by flow cytometry, and transcriptionally upregulated key transcription factors in p53/p21-mediated cell cycle pathways. Moreover, compared to the control condition, triisobutyl phosphate and trimethyl phosphate exposure increased the sub-G1 apoptotic peak and upregulated the p53/ bax apoptosis pathway, indicating potential cell apoptosis at the cellular and molecular levels. These results provide insight into PFR toxicity and the involved mode of action and indicate the mitochondria is an important target for some alkyl-PFRs. (C) 2020 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45312]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Yuan, Shengwu,Zhu, Kongrui,Ma, Mei,et al. In vitro oxidative stress, mitochondrial impairment and G1 phase cell cycle arrest induced by alkyl-phosphorus-containing flame retardants[J]. CHEMOSPHERE,2020,248:1-9. |
| APA | Yuan, Shengwu,Zhu, Kongrui,Ma, Mei,Zhu, Xiaoshan,Rao, Kaifeng,&Wang, Zijian.(2020).In vitro oxidative stress, mitochondrial impairment and G1 phase cell cycle arrest induced by alkyl-phosphorus-containing flame retardants.CHEMOSPHERE,248,1-9. |
| MLA | Yuan, Shengwu,et al."In vitro oxidative stress, mitochondrial impairment and G1 phase cell cycle arrest induced by alkyl-phosphorus-containing flame retardants".CHEMOSPHERE 248(2020):1-9. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。