Screening of chemicals with binding activities of liver X receptors from reclaimed waters
文献类型：期刊论文
| 作者 | Zhang, Haifeng; Jia, Yingting; Tang, Zhuoheng; Wang, Lei; Hu, Wenxin; Gao, Junmin; Hu, Jianying; Yang, Min |
| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2020-04-15 |
| 卷号 | 713页码:1-59 |
| 关键词 | Endocrine disrupting chemicals Liver X receptors Reclaimed wastewater Nuclear receptors binding extraction High resolution mass spectrometry |
| ISSN号 | 0048-9697 |
| 英文摘要 | Wastewater reclamation and reuse is considered an attractive and practical method for coping with water scarcity. However, the presence of micropollutants in reclaimed water, including endocrine disrupting chemicals (EDCs), is a major public health concern. This study attempted to identify unknown EDCs with liver X receptor (LXR alpha) agonist/antagonist activities in reclaimed wastewater, using nuclear receptors binding extraction coupled with high-resolution mass spectrometry (NRBE-HRMS). In total, 105 compounds in the reclaimed wastewater exhibited LXR alpha-binding activity. Among them, two previously unknown LXR alpha-antagonist compounds, catechol and 4-acetamidoantipyrine, were identified, based on authentic standards. The two LXR alpha-antagonist compounds exhibited weak LXR alpha-antagonist activities in a yeast two-hybrid assay. Catechol and 4-acetamidoantipyrine inhibited the beta-galactosidase activity induced by 60 nM of TO901317 in an LXR alpha yeast assay, with IC20 values of 79,938.9 nM and 6286.4 nM, respectively. To the best of our knowledge, this is the first study to identify EDCs in reclaimed wastewater with LXR alpha-agonist/antagonist activity using the NRBE-HRMS method. (C) 2020 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45360]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhang, Haifeng,Jia, Yingting,Tang, Zhuoheng,et al. Screening of chemicals with binding activities of liver X receptors from reclaimed waters[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,713:1-59. |
| APA | Zhang, Haifeng.,Jia, Yingting.,Tang, Zhuoheng.,Wang, Lei.,Hu, Wenxin.,...&Yang, Min.(2020).Screening of chemicals with binding activities of liver X receptors from reclaimed waters.SCIENCE OF THE TOTAL ENVIRONMENT,713,1-59. |
| MLA | Zhang, Haifeng,et al."Screening of chemicals with binding activities of liver X receptors from reclaimed waters".SCIENCE OF THE TOTAL ENVIRONMENT 713(2020):1-59. |
入库方式： OAI收割
来源：生态环境研究中心
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