The steroid receptor coactivator 1 (SRC1) and 3 (SRC3) recruitment as novel molecular initiating event of 4-n-nonylphenol in estrogen receptor alpha-mediated pathways
文献类型：期刊论文
| 作者 | Ji, Xiaoya; Li, Na ; Yang, Rong; Rao, Kaifeng; Ma, Mei; Wang, Zijian
|
| 刊名 | ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
 |
| 出版日期 | 2020-02 |
| 卷号 | 189页码:1-8 |
| 关键词 | 4-n-Nonylphenol Estrogenic activity Molecular initiating event (MIE) Estrogen receptor alpha (ER alpha) Steroid receptor coactivator (SRC) |
| ISSN号 | 0147-6513 |
| 英文摘要 | Recently, the action of steroid receptor coactivators (SRCs) has been recognized to be an important molecular initiating event (MIE) in estrogenic adverse outcome pathways (AOPs). However, the role of SRCs in the molecular mechanisms of many highly concerned environmental estrogens remains poorly understood. In this study, the widely studied environmental estrogen, 4-n-nonylphenol (4-n-NP), was used as a typical pollutant to study SRCs recruitment in its estrogenic effects. In MCF7 cell proliferation (E-SCREEN) assay and MVLN cell assay, 4-n-NP showed significant estrogenic potency that involved an increase in estrogen receptor alpha (ER alpha), SRC1 and SRC3 transcript levels. Moreover, 4-n-NP was found to induce estrogen response element (ERE)-mediated activity via ER alpha in MVLN cells. To investigate the mechanism by which SRCs recruitment is induced by 4-n-NP-ER alpha, a coactivators recruitment assay was performed, and the results showed that 4-n-NP-ER alpha recruited both SRC1 and SRC3, whereas it failed to recruit SRC2. Similarly, it had no interaction with SRC2 in the ER alpha-SRC2 two-hybrid yeast assay. This is the first report to investigate the novel MIE of SRCs recruitment in 4-n-NP-ER alpha-induced estrogenicity. Overall, our results suggest that the action of 4-n-NP on estrogenic effects involves the following MIEs: the activation of ER alpha, the recruitment of SRC1 and SRC3, and the induction of ERE-mediated activity. The findings also provide valuable insights into the MIE associated with the different SRCs that are recruited in the adverse outcome pathways of environmental estrogens. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/45370]  |
| 专题 | 生态环境研究中心_中国科学院饮用水科学与技术重点实验室 |
| 推荐引用方式 GB/T 7714 | Ji, Xiaoya,Li, Na,Yang, Rong,et al. The steroid receptor coactivator 1 (SRC1) and 3 (SRC3) recruitment as novel molecular initiating event of 4-n-nonylphenol in estrogen receptor alpha-mediated pathways[J]. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,2020,189:1-8. |
| APA | Ji, Xiaoya,Li, Na,Yang, Rong,Rao, Kaifeng,Ma, Mei,&Wang, Zijian.(2020).The steroid receptor coactivator 1 (SRC1) and 3 (SRC3) recruitment as novel molecular initiating event of 4-n-nonylphenol in estrogen receptor alpha-mediated pathways.ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY,189,1-8. |
| MLA | Ji, Xiaoya,et al."The steroid receptor coactivator 1 (SRC1) and 3 (SRC3) recruitment as novel molecular initiating event of 4-n-nonylphenol in estrogen receptor alpha-mediated pathways".ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY 189(2020):1-8. |
入库方式： OAI收割
来源：生态环境研究中心
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。