Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo
文献类型：期刊论文
| 作者 | Cao, Nan-Jue1,3; Zhu, Yu-He1; Gao, Fei1; Liang, Chen2; Wang, Zhen-Bo2; Zhang, Yue1; Hao, Chun-Ping1; Wang, Wei1 |
| 刊名 | ANNALS OF TRANSLATIONAL MEDICINE
 |
| 出版日期 | 2021-04-01 |
| 卷号 | 9期号:7页码:15 |
| 关键词 | Surface mechanical attrition treatment (SMAT) titanium (Ti) nanostructure bone mesenchymal stem cells (BMSCs) osseointegration |
| ISSN号 | 2305-5839 |
| DOI | 10.21037/atm-20-7588 |
| 通讯作者 | Wang, Wei(wwang75@cmu.edu.cn) |
| 英文摘要 | Background: Though titanium (Ti) is widely used as dental materials in the clinic, effective methods to treat Ti for higher surface biological activity still lack. Through Surface mechanical attrition treatment (SMAT) technology we could endow Ti with gradient nanostructured surface (GNS Ti). To investigate the biocompatibility of GNS Ti for its further application in dental implant field, we study the effects of GNS Ti on cell responses in vitro and osseointegration of the implant with surrounding bone tissues in vivo. Methods: In this study, GNS Ti was fabricated by SMAT. In vitro experiment, we co-cultured GNS Ti with bone mesenchymal stem cells (BMSCs), surface characterization was detected by transmission electron microscope (TEM). Adhesion, proliferation and differentiation of BMSCs were evaluated by scanning electron microscope (SEM), MTT, flow cytometry (FCM), alkaline phosphatase (ALP) and osteocalcin (OCN) tests. in vivo experiment, the GNS Ti was implanted into the rabbit mandible. Osteogenesis and osseointegration were evaluated by Micro CT, toluidine blue staining, and immunohistochemical staining at 4, 8, and 12 weeks postoperatively. Results: Both results showed that compared with the coarse grained (CG) Ti, the GNS Ti stimulated the adhesion, proliferation, and differentiation of BMSCs and improved osteogenesis and osseointegration. Conclusions: This study indicates that gradient nanostructured Ti is a promising material for dental implant application. |
| 资助项目 | National Natural Science Foundation of China[81970980] ; Liaoning Provincial Key Research Plan Guidance Project[2018225078] ; Liaoning Provincial Natural Science Foundation Guidance Project[2019ZD0749] ; Central Government of Liaoning Province to Guide Local Science and Technology Development Project[2017108001] ; Shenyang Major Scientific and Technological Innovation Research and Development Plan[191124027] ; Shenyang Young and Middleaged Technological Innovation Talent Plan[RC200060] |
| WOS研究方向 | Oncology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000641416400001 |
| 出版者 | AME PUBL CO |
| 资助机构 | National Natural Science Foundation of China ; Liaoning Provincial Key Research Plan Guidance Project ; Liaoning Provincial Natural Science Foundation Guidance Project ; Central Government of Liaoning Province to Guide Local Science and Technology Development Project ; Shenyang Major Scientific and Technological Innovation Research and Development Plan ; Shenyang Young and Middleaged Technological Innovation Talent Plan |
| 源URL | [http://ir.imr.ac.cn/handle/321006/161649]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 通讯作者 | Wang, Wei |
| 作者单位 | 1.China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, 117 Nanjing North St, Shenyang 110002, Peoples R China 2.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang, Peoples R China 3.Zhejiang Univ, Affiliated Hosp 4, Sch Med, Yiwu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Nan-Jue,Zhu, Yu-He,Gao, Fei,et al. Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo[J]. ANNALS OF TRANSLATIONAL MEDICINE,2021,9(7):15. |
| APA | Cao, Nan-Jue.,Zhu, Yu-He.,Gao, Fei.,Liang, Chen.,Wang, Zhen-Bo.,...&Wang, Wei.(2021).Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo.ANNALS OF TRANSLATIONAL MEDICINE,9(7),15. |
| MLA | Cao, Nan-Jue,et al."Gradient nanostructured titanium stimulates cell responses in vitro and enhances osseointegration in vivo".ANNALS OF TRANSLATIONAL MEDICINE 9.7(2021):15. |
入库方式： OAI收割
来源：金属研究所
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