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Dysregulation of ClpP by Small-Molecule Activators Used Against Xanthomonas oryzae pv. oryzae Infections

文献类型：期刊论文

作者Yang, Teng1,2; Zhang, Tao2; Zhou, Xiang1; Wang, Pengyu2,3; Gan, Jianhua4; Song, Baoan1; Yang, Song1; Yang, Cai-Guang2,3,5
刊名JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
出版日期2021-07-14
卷号69期号:27页码:7545-7553
关键词rice bacterial leaf blight caseinolytic protease P (ClpP) filamentous temperature-sensitive Z (FtsZ) ONC212 ADEP4 activator anti-Xoo infection
ISSN号0021-8561
DOI10.1021/acs.jafc.1c01470
通讯作者Yang, Song(jhzx.msm@gmail.com) ; Yang, Cai-Guang(yangcg@simm.ac.cn)
英文摘要Rice bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is considered a destructive plant bacterial disease. The looming crisis of antibiotic resistance necessitates the discovery of antibiotics with new modes of action. Activated caseinolytic protease P (ClpP) can degrade bacterial FtsZ proteins that are essential for cell division; thus, we hypothesized that small-molecule-induced dysregulation of XooClpP may result in degradation of XooFtsZ to treat leaf blight diseases. In this work, we have determined the crystal structures of XooClpP, and its mutant bound with ADEP4, which revealed the action modes of XooClpP assemblies and XooFtsZ degradation by dysregulated XooClpP in the presence of small-molecule activators, such as ONC212 and ADEP4. Additionally, an antibacterial assessment demonstrated that ONC212 displays excellent activity against Xoo and prevents rice bacterial leaf blight in vivo. Thus, these unique antibacterial effects of small-molecule activators of XooClpP represent a potential strategy for the development of agricultural antibiotics by targeting bacterial ClpP.
WOS关键词STAPHYLOCOCCUS-AUREUS ; PROTEASE ; FTSZ ; RESISTANCE ; VIRULENCE ; BACTERIA ; POTENT
资助项目National Natural Science Foundation of China[22037007] ; National Natural Science Foundation of China[21877021] ; National Natural Science Foundation of China[21725801]
WOS研究方向Agriculture ; Chemistry ; Food Science & Technology
语种英语
WOS记录号WOS:000674324600003
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/296756]  
专题新药研究国家重点实验室
通讯作者Yang, Song; Yang, Cai-Guang
作者单位1.Guizhou Univ, Ctr R&D Fine Chem, State Key Lab Breeding Base Green Pesticide & Agr, Key Lab Green Pesticide & Agr Bioengn,Minist Educ, Guiyang 550025, Peoples R China
2.Chinese Acad Sci, Ctr Chem Biol, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China
5.UCAS, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Teng,Zhang, Tao,Zhou, Xiang,et al. Dysregulation of ClpP by Small-Molecule Activators Used Against Xanthomonas oryzae pv. oryzae Infections[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2021,69(27):7545-7553.
APA Yang, Teng.,Zhang, Tao.,Zhou, Xiang.,Wang, Pengyu.,Gan, Jianhua.,...&Yang, Cai-Guang.(2021).Dysregulation of ClpP by Small-Molecule Activators Used Against Xanthomonas oryzae pv. oryzae Infections.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,69(27),7545-7553.
MLA Yang, Teng,et al."Dysregulation of ClpP by Small-Molecule Activators Used Against Xanthomonas oryzae pv. oryzae Infections".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 69.27(2021):7545-7553.

入库方式： OAI收割

来源：上海药物研究所

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