Simultaneous determination of monocrotaline and its N-oxide metabolite in rat plasma using LC-MS/MS: Application to a pharmacokinetic study
文献类型：期刊论文
| 作者 | Lin, Feifei1,2 ; Pan, Anni2; Ye, Yang1,2; Liu, Jia2
|
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2021-07-08 |
| 页码 | 7 |
| 关键词 | LC-MS MS monocrotaline monocrotaline N-oxide pharmacokinetics |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.5207 |
| 通讯作者 | Ye, Yang(yye@simm.ac.cn) ; Liu, Jia(jia.liu@simm.ac.cn) |
| 英文摘要 | Monocrotaline (MCT) is a pyrrolizidine alkaloid that can induce hepatic sinusoidal damage, pulmonary hypertension, renal toxicity, and heart disease. Monocrotaline N-oxide (MNO), the primary metabolite of MCT, is less toxic; however, it can convert back to MCT to exhibit its toxicity. This study developed and validated a rapid and sensitive LC-MS/MS method for the simultaneous determination of MCT and monocrotaline N-oxide in rat plasma. The method has a linearity over the concentration range of 1-2000 ng/mL with correlation coefficients (r) >0.997 for each analyte. The results of selectivity, matrix effect, accuracy and precision, and recovery were all within the acceptance criteria. The validated method has been successfully applied to study pharmacokinetic behaviors and bioavailability of MCT in rats. MCT was rapidly absorbed (T-max: 0.400 +/- 0.149 h) after oral administration, and the absolute bioavailability of MCT was 78.2%. |
| WOS关键词 | PYRROLIZIDINE ALKALOIDS ; ACTIVATION ; TOXICITY ; LIVER |
| 资助项目 | National Natural Science Foundation of China[21920102003] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000670795900001 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/296952]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Ye, Yang; Liu, Jia |
| 作者单位 | 1.Nanjing Univ Chinese Med, Sch Chinese Mat Med, 138 Xianlin Ave, Nanjing 210023, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lin, Feifei,Pan, Anni,Ye, Yang,et al. Simultaneous determination of monocrotaline and its N-oxide metabolite in rat plasma using LC-MS/MS: Application to a pharmacokinetic study[J]. BIOMEDICAL CHROMATOGRAPHY,2021:7. |
| APA | Lin, Feifei,Pan, Anni,Ye, Yang,&Liu, Jia.(2021).Simultaneous determination of monocrotaline and its N-oxide metabolite in rat plasma using LC-MS/MS: Application to a pharmacokinetic study.BIOMEDICAL CHROMATOGRAPHY,7. |
| MLA | Lin, Feifei,et al."Simultaneous determination of monocrotaline and its N-oxide metabolite in rat plasma using LC-MS/MS: Application to a pharmacokinetic study".BIOMEDICAL CHROMATOGRAPHY (2021):7. |
入库方式： OAI收割
来源：上海药物研究所
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