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Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography

文献类型：期刊论文

作者Jiang, Liyuan1,2,3,5,6; Li, Chengjun1,2,4,5,6; Li, Miao1,2,4,5,6; Yin, Xianzhen7; Wu, Tianding1,2,4,5,6; Duan, Chunyue1,2,4,5,6; Cao, Yong1,2,4,5,6; Lu, Hongbin3,4,5,6; Hu, Jianzhong1,2,3,5,6
刊名NEUROSCIENCE BULLETIN
出版日期2021-06-19
页码12
关键词Sr mu ct 3D High-resolution Neurovascular Spinal cord
ISSN号1673-7067
DOI10.1007/s12264-021-00715-7
通讯作者Cao, Yong(caoyong1912@163.com) ; Hu, Jianzhong(jianzhonghu@hotmail.com)
英文摘要Effective methods for visualizing neurovascular morphology are essential for understanding the normal spinal cord and the morphological alterations associated with diseases. However, ideal techniques for simultaneously imaging neurovascular structure in a broad region of a specimen are still lacking. In this study, we combined Golgi staining with angiography and synchrotron radiation micro-computed tomography (SR mu CT) to visualize the 3D neurovascular network in the mouse spinal cord. Using our method, the 3D neurons, nerve fibers, and vasculature in a broad region could be visualized in the same image at cellular resolution without destructive sectioning. Besides, we found that the 3D morphology of neurons, nerve fiber tracts, and vasculature visualized by SR mu CT were highly consistent with that visualized using the histological method. Moreover, the 3D neurovascular structure could be quantitatively evaluated by the combined methodology. The method shown here will be useful in fundamental neuroscience studies.
WOS关键词PHASE-CONTRAST TOMOGRAPHY ; BRAIN ; HISTOLOGY ; CELLS ; CT
资助项目National Natural Science Foundation of China[82030071] ; National Natural Science Foundation of China[81874004] ; National Natural Science Foundation of China[81672174] ; Key R&D Program of the Hunan Provincial Science & Technology Department[2017SK2061] ; Hunan Provincial Department of Finance[(2018)2] ; Fundamental Research Funds for the Central Universities of Central South University[2018zzts254]
WOS研究方向Neurosciences & Neurology
语种英语
WOS记录号WOS:000663495900001
出版者SPRINGER
源URL[http://119.78.100.183/handle/2S10ELR8/297128]  
专题中国科学院上海药物研究所
通讯作者Cao, Yong; Hu, Jianzhong
作者单位1.Cent South Univ, Xiangya Hosp, Dept Spine Surg, Changsha 410008, Peoples R China
2.Cent South Univ, Xiangya Hosp, Natl Clin Res Ctr Geriatr Disorders, Changsha 410008, Peoples R China
3.Key Lab Organ Injury Aging & Regenerat Med Hunan, Changsha 410008, Peoples R China
4.Cent South Univ, Xiangya Hosp, Dept Sports Med, Changsha 410008, Peoples R China
5.Xiangya Hosp, Int Chinese Musculoskeletal Res Soc Sports Med Re, Changsha 410008, Peoples R China
6.Hunan Engn Res Ctr Sport & Hlth, Changsha 410008, Peoples R China
7.Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Jiang, Liyuan,Li, Chengjun,Li, Miao,et al. Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography[J]. NEUROSCIENCE BULLETIN,2021:12.
APA Jiang, Liyuan.,Li, Chengjun.,Li, Miao.,Yin, Xianzhen.,Wu, Tianding.,...&Hu, Jianzhong.(2021).Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography.NEUROSCIENCE BULLETIN,12.
MLA Jiang, Liyuan,et al."Simultaneous 3D Visualization of the Microvascular and Neural Network in Mouse Spinal Cord Using Synchrotron Radiation Micro-Computed Tomography".NEUROSCIENCE BULLETIN (2021):12.

入库方式： OAI收割

来源：上海药物研究所

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