Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS
文献类型：期刊论文
| 作者 | Wei Wen-Long; Wu Shi-Fei; Li Hao-Jv; Li Zhen-Wei; Qu Hua; Yao Chang-Liang; Zhang Jian-Qing; Li Jia-Yuan; Wu Wan-Ying ; Guo De-An
|
| 刊名 | CHINESE JOURNAL OF NATURAL MEDICINES
 |
| 出版日期 | 2021-06-01 |
| 卷号 | 19期号:6页码:473-480 |
| 关键词 | Huashi Baidu prescription Corona virus disease 2019 Characterization of chemical constituents ELISA |
| ISSN号 | 2095-6975 |
| DOI | 10.1016/S1875-5364(21)60046-8 |
| 通讯作者 | Guo De-An(gda5958@163.com) |
| 英文摘要 | Huashi Baidu prescription (HSBDF), recommended in the Guideline for the Diagnosis and Treatment of Novel Coronavirus (2019-nCoV) Pneumonia (On Trials, the Seventh Edition), was clinically used to treat severe corona virus disease 2019 (COVID-19) with cough, blood-stained sputum, inhibited defecation, red tongue etc. symptoms. This study was aimed to elucidate and profile the knowledge on its chemical constituents and the potential anti-inflammatory effect in vitro. In the study, the chemical constituents in extract of HSBDF were characterized by UPLC-Q-TOF/MS in both negative and positive modes, and the pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assays (ELISA) to determine the effects of HSBDF in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results showed that a total of 217 chemical constituents were tentativedly characterized in HSBDF. Moreover, HSBDF could alleviate the expression levels of IL-6 and TNF-alpha in the cell models, indicating that the antiviral effects of HSBDF might be associated with regulation of the inflammatory cytokines production in RAW264.7 cells. We hope that the results could be served as the basic data for further study of HSBDF on anti-COVID-19 effect. |
| WOS关键词 | FLIGHT MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; QUADRUPOLE-TIME ; PHENOLIC PROFILES ; RADIX ASTRAGALI ; RHEUM-PALMATUM ; RAT PLASMA ; CONSTITUENTS ; IDENTIFICATION ; METABOLITES |
| 资助项目 | National Key R&D Program of China[2019YFC1711000] |
| WOS研究方向 | Integrative & Complementary Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000659173300008 |
| 出版者 | CHINESE JOURNAL NATURAL MEDICINES |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/297416]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo De-An |
| 作者单位 | Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei Wen-Long,Wu Shi-Fei,Li Hao-Jv,et al. Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2021,19(6):473-480. |
| APA | Wei Wen-Long.,Wu Shi-Fei.,Li Hao-Jv.,Li Zhen-Wei.,Qu Hua.,...&Guo De-An.(2021).Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS.CHINESE JOURNAL OF NATURAL MEDICINES,19(6),473-480. |
| MLA | Wei Wen-Long,et al."Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS".CHINESE JOURNAL OF NATURAL MEDICINES 19.6(2021):473-480. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。