Novel Plasma miRNAs as Biomarkers and Therapeutic Targets of Alzheimer's Disease at the Prodromal Stage
文献类型：期刊论文
| 作者 | He, Haining1,2; Liu, An1,3; Zhang, Wei1,3; Yang, Huanqing1,3; Zhang, Minmin4 ; Xu, Hua1,3; Liu, Yuanyuan1,3; Hong, Bo1,3; Yan, Feng1,3; Yue, Ling1,3
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| 刊名 | JOURNAL OF ALZHEIMERS DISEASE
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| 出版日期 | 2021 |
| 卷号 | 83期号:2页码:779-790 |
| 关键词 | Alzheimer's disease biomarkers microRNAs mild cognitive impairment |
| ISSN号 | 1387-2877 |
| DOI | 10.3233/JAD-210307 |
| 通讯作者 | Xiao, Shifu(xiaoshifu@msn.com) ; Xie, Zuoquan(zqxie@simm.ac.cn) ; Wang, Tao(wtshhwy@163.com) |
| 英文摘要 | Background: Amnestic mild cognitive impairment (aMCI) is a prodromal stage of Alzheimer's disease (AD) involving imbalanced beta-site amyloid precursor protein cleaving enzyme 1 (BACE1). MicroRNAs (miRNAs) are associated with AD. Objective: This study aimed to investigated whether plasma miRNAs can predict prodromal AD or are associated with AD pathology. Methods: Participants in the discovery set (n = 10), analysis set (n = 30), and validation set (n = 80) were screened from the China Longitudinal Aging Study. RNA was extracted from the participants' plasma. Microarray sequencing provided miRNA profiles and differentially expressed miRNAs (DEmiRNAs) in the discovery set included patients with F-18-Flutemetamol positron emission tomography scan-confirmed aMCI. Potential biomarkers were screened in the analysis set. The predict capability of candidate miRNAs was assessed in the validation set. Candidate miRNAs modulation of BACE1 expression was explored in rat and human hippocampal neurons in vitro. Results: We verified 46 significant DEmiRNAs between the aMCI and NC groups (p < 0.05), among which 33 were downregulated. In the analysis set, miR-1185-2-3p, miR-1909-3p, miR-22-5p, and miR-134-3p levels decreased significantly in the aMCI group. These miRNAs and previously identified miR-107 were selected as potential biomarkers. A prediction model comprising these five miRNAs showed outstanding accuracy (81.25%) to discriminate aMCI at cut-off value of 0.174. Except for miR-134-3p, the other four miRNAs significantly suppressed Bace1 expression in rat hippocampal neurons in vitro. BACE1 modulation of miR-1185-2-3p, miR-1909-3p, and miR-134-3p was confirmed in human hippocampal neurons in vitro. Conclusion: A predictive model consisting of five BACE1-related plasma miRNAs could be a novel biomarker for a MCI. |
| WOS关键词 | MILD COGNITIVE IMPAIRMENT ; GENE-EXPRESSION ; A-BETA ; PREDICTION ; DIAGNOSIS ; ASSOCIATION ; VALIDATION ; PLASTICITY ; MICRORNAS ; DEMENTIA |
| 资助项目 | Clinical Research Center, Shanghai Mental Health Center[CRC2019ZD03] ; Clinical Research Center, Shanghai Mental Health Center[CRC2017ZD02] ; National Natural Science Foundation of China[81571298] ; Shanghai Clinical Research Center for Mental Health[19MC1911100] ; Science and Technology Commission of Shanghai Municipality[18411961500] ; Shanghai Health System Excellent Talent Training Program[2017BR054] |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000696383200024 |
| 出版者 | IOS PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/297507]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Xiao, Shifu; Xie, Zuoquan; Wang, Tao |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Shanghai Mental Hlth Ctr, Dept Geriatr Psychiat, Sch Med, Shanghai, Peoples R China 2.Sun Yat Sen Univ, Dept Psychiat, Affiliated Hosp 3, Guangzhou, Peoples R China 3.Shanghai Jiao Tong Univ, Alzheimers Dis & Related Disorders Ctr, Shanghai, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Haining,Liu, An,Zhang, Wei,et al. Novel Plasma miRNAs as Biomarkers and Therapeutic Targets of Alzheimer's Disease at the Prodromal Stage[J]. JOURNAL OF ALZHEIMERS DISEASE,2021,83(2):779-790. |
| APA | He, Haining.,Liu, An.,Zhang, Wei.,Yang, Huanqing.,Zhang, Minmin.,...&Wang, Tao.(2021).Novel Plasma miRNAs as Biomarkers and Therapeutic Targets of Alzheimer's Disease at the Prodromal Stage.JOURNAL OF ALZHEIMERS DISEASE,83(2),779-790. |
| MLA | He, Haining,et al."Novel Plasma miRNAs as Biomarkers and Therapeutic Targets of Alzheimer's Disease at the Prodromal Stage".JOURNAL OF ALZHEIMERS DISEASE 83.2(2021):779-790. |
入库方式： OAI收割
来源：上海药物研究所
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