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Structural basis for genome packaging, retention, and ejection in human cytomegalovirus

文献类型：期刊论文

作者Li, Zhihai1; Pang, Jingjing1,2; Dong, Lili1; Yu, Xuekui1,2
刊名NATURE COMMUNICATIONS
出版日期2021-07-27
卷号12期号:1页码:14
ISSN号2041-1723
DOI10.1038/s41467-021-24820-3
通讯作者Yu, Xuekui(xkyu@simm.ac.cn)
英文摘要How the human cytomegalovirus (HCMV) genome-the largest among human herpesviruses-is packaged, retained, and ejected remains unclear. We present the in situ structures of the symmetry-mismatched portal and the capsid vertex-specific components (CVSCs) of HCMV. The 5-fold symmetric 10-helix anchor-uncommon among known portals-contacts the portal-encircling DNA, which is presumed to squeeze the portal as the genome packaging proceeds. We surmise that the 10-helix anchor dampens this action to delay the portal reaching a "head-full" packaging state, thus facilitating the large genome to be packaged. The 6-fold symmetric turret, latched via a coiled coil to a helix from a major capsid protein, supports the portal to retain the packaged genome. CVSCs at the penton vertices-presumed to increase inner capsid pressure-display a low stoichiometry, which would aid genome retention. We also demonstrate that the portal and capsid undergo conformational changes to facilitate genome ejection after viral cell entry. Human cytomegalovirus (HCMV) is the prototypical member of the beta -herpesvirinae subfamily and the leading viral cause of congenital infections that can lead to birth defects and it can also cause life-threatening disease in immunocompromised individuals. Here, the authors present the in-situ cryo-EM structures of the symmetry-mismatched portal and the capsid vertex-specific components (CVSCs) of HCMV and discuss the mechanistic implications for genome package, retention and ejection.
WOS关键词HERPES-SIMPLEX ; TERMINASE SUBUNITS ; DNA ; PROTEIN ; INFECTION ; MANIFESTATIONS ; PREDICTION ; STABILITY ; CLEAVAGE ; COMPLEX
资助项目100 Talents Program of the Chinese Academy of Sciences ; Natural Science Foundation of Shanghai[18ZR1447700] ; National Natural Science Foundation of China[31900869] ; Shanghai Sailing Program[19YF1456800]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000683367300001
出版者NATURE PORTFOLIO
源URL[http://119.78.100.183/handle/2S10ELR8/297661]  
专题中国科学院上海药物研究所
通讯作者Yu, Xuekui
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Cryoelectron Microscopy Res Ctr, Shanghai, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Li, Zhihai,Pang, Jingjing,Dong, Lili,et al. Structural basis for genome packaging, retention, and ejection in human cytomegalovirus[J]. NATURE COMMUNICATIONS,2021,12(1):14.
APA Li, Zhihai,Pang, Jingjing,Dong, Lili,&Yu, Xuekui.(2021).Structural basis for genome packaging, retention, and ejection in human cytomegalovirus.NATURE COMMUNICATIONS,12(1),14.
MLA Li, Zhihai,et al."Structural basis for genome packaging, retention, and ejection in human cytomegalovirus".NATURE COMMUNICATIONS 12.1(2021):14.

入库方式： OAI收割

来源：上海药物研究所

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