Fast determination of 16 circulating neurotransmitters and their metabolites in plasma samples of spontaneously hypertensive rats intervened with five different Uncaria
文献类型：期刊论文
| 作者 | Gao, Lei1,2; Zhang, Zijia1; Feng, Zijin1; Wei, Wenlong1; Wu, Wenyong1; Zhi, Haijuan1; Deng, Yanping1; Long, Huali1; Lei, Min1 ; Hou, Jinjun1
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| 出版日期 | 2021-08-01 |
| 卷号 | 1179页码:9 |
| ISSN号 | 1570-0232 |
| 关键词 | Neurotransmitters LC-MS Hypertension Uncaria Spontaneously hypertensive rats |
| DOI | 10.1016/j.jchromb.2021.122856 |
| 通讯作者 | Guo, De-an(daguo@simm.ac.cn) ; Wu, Wanying(wanyingwu@simm.ac.cn) |
| 英文摘要 | This study aimed to establish a sensitive, reproducible, and rapid liquid chromatography method with tandem mass spectrometry detection to perform simultaneous quantitative analysis of 16 neurotransmitters and their metabolites in rat plasma, including levodopa, dopamine, norepinephrine, epinephrine, L-tryptophan, kynurenic acid, serotonin, melatonin, choline, acetylcholine, histamine, phenylethylamine, as well as excitatory (L-glutamic acid and L-aspartic acid) and inhibitory (gamma-aminobutyric acid and L-glycine) neurotransmitters. These analytes were measured by ultra-high performance chromatography coupled with triple quadrupole mass spectrometry using a hydrophilic interaction chromatographic column (ethylene-bridged hybrid amide column). The internal standards of stable isotope labeling were used to improve the reliability of the results. Our method provided high linearity for all neurotransmitters (for all coefficients measured > 0.99), with inter- and intra-day accuracy from -14.82% to 17.49% and precision was between 0.89% and 17.70%. The method was subsequently verified in an animal study, where the intervention of five different Uncarias, the traditional Chinese medicine with hypotensive effects, was applied to the spontaneously hypertensive rats (SHRs). SHRs showed dysregulated plasma kynurenic acid, acetylcholine, and norepinephrine levels, and these neuroactive analytes were significantly restored by Uncaria treatment compared with the model group (SHR group). Compared with captopril, included as a positive control for its hypotensive effect, Uncaria had more effects on perturbing the levels of plasma neurotransmitters, which might indicate Uncaria's potential in treating symptoms related to the nervous system. These results suggested that the changes in the neurotransmitters and their metabolites in plasma may be related to the pathogenesis of hypertension. It also provided valuable information about the action mechanisms of Uncaria on its hypotensive effects. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; MONOAMINE NEUROTRANSMITTERS ; ONLINE DERIVATIZATION ; AMINO-ACIDS ; MICRODIALYSIS ; BRAIN ; METABOLOMICS |
| 资助项目 | National Natural Science Foundation of China[81673591] ; National Key R&D Program of China[2018YFC1707900] ; National Key R&D Program of China[2018YFC1707001] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000687831200016 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/297712]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, De-an; Wu, Wanying |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Haike Rd 501, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuanquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Lei,Zhang, Zijia,Feng, Zijin,et al. Fast determination of 16 circulating neurotransmitters and their metabolites in plasma samples of spontaneously hypertensive rats intervened with five different Uncaria[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2021,1179:9. |
| APA | Gao, Lei.,Zhang, Zijia.,Feng, Zijin.,Wei, Wenlong.,Wu, Wenyong.,...&Wu, Wanying.(2021).Fast determination of 16 circulating neurotransmitters and their metabolites in plasma samples of spontaneously hypertensive rats intervened with five different Uncaria.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1179,9. |
| MLA | Gao, Lei,et al."Fast determination of 16 circulating neurotransmitters and their metabolites in plasma samples of spontaneously hypertensive rats intervened with five different Uncaria".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1179(2021):9. |
入库方式： OAI收割
来源：上海药物研究所
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