Application of L-Threonine Aldolase to on-DNA Reactions
文献类型：期刊论文
| 作者 | Chai, Jing1; Lu, Xiaojie1,2,3 ; Arico-Muendel, Christopher C.1; Ding, Yun1; Pollastri, Michael P.4
|
| 刊名 | BIOCONJUGATE CHEMISTRY
 |
| 出版日期 | 2021-09-15 |
| 卷号 | 32期号:9页码:1973-1978 |
| ISSN号 | 1043-1802 |
| DOI | 10.1021/acs.bioconjchem.1c00363 |
| 通讯作者 | Chai, Jing(jing.5.chai@gsk.com) |
| 英文摘要 | Enzymatic catalysis is a highly attractive approach to the DNA encoded library technology (DEL) that has not been widely explored. In this paper, we report an L-threonine aldolase (L-TA)-catalyzed on-DNA aldol reaction to form beta-hydroxy-alpha-amino acids, and its diastereoselectivity determination. L-TAs from three species show good on-DNA aldehyde scope and complementary stereoselectivity. The formed aldol product can be further diversified via various reactions, which demonstrates the utility of this reaction in DEL. |
| WOS关键词 | ENCODED CHEMICAL LIBRARIES ; DRUG DISCOVERY ; SUBSTRATE ; SPECIFICITY ; TECHNOLOGY ; SELECTION ; ACIDS ; TOOL |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000697280800005 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/297884]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Chai, Jing |
| 作者单位 | 1.GlaxoSmithKline, R&D Med Sci & Technol, Encoded Lib Technol NCE Mol Discovery, Cambridge, MA 02140 USA 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Northeastern Univ, Dept Chem & Chem Biol, Boston, MA 02115 USA |
| 推荐引用方式 GB/T 7714 | Chai, Jing,Lu, Xiaojie,Arico-Muendel, Christopher C.,et al. Application of L-Threonine Aldolase to on-DNA Reactions[J]. BIOCONJUGATE CHEMISTRY,2021,32(9):1973-1978. |
| APA | Chai, Jing,Lu, Xiaojie,Arico-Muendel, Christopher C.,Ding, Yun,&Pollastri, Michael P..(2021).Application of L-Threonine Aldolase to on-DNA Reactions.BIOCONJUGATE CHEMISTRY,32(9),1973-1978. |
| MLA | Chai, Jing,et al."Application of L-Threonine Aldolase to on-DNA Reactions".BIOCONJUGATE CHEMISTRY 32.9(2021):1973-1978. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。