Rapid nucleic acid detection of Escherichia coli O157:H7 based on CRISPR/ Cas12a system
文献类型：期刊论文
| 作者 | Wang, Shujuan1; Fan, Yiling1; Feng, Zhen1; Song, Minghui1; Li, Qiongqiong1; Jiang, Bo1; Qin, Feng1; Liu, Hao1; Lan, Lefu2 ; Yang, Meicheng1
|
| 刊名 | FOOD CONTROL
 |
| 出版日期 | 2021-12-01 |
| 卷号 | 130页码:8 |
| ISSN号 | 0956-7135 |
| 关键词 | CRISPR/Cas12a Fe3O4@PDA@UiO-66-NH2 Nucleic acid Detection Escherichia coli O157:H7 |
| DOI | 10.1016/j.foodcont.2021.108194 |
| 通讯作者 | Yang, Meicheng(yangmeicheng@vip.sina.com) |
| 英文摘要 | Escherichia coli O157:H7 (E. coli O157:H7) is one of the most dangerous foodborne pathogens causing severe illnesses in the elderly, children and immunocompromised people. Traditional methods could not meet the detection requirements of high speed, sensitivity, and specificity. The clustered regularly interspaced short palindromic repeats (CRISPR)-based diagnostic is a novel high sensitivity and specificity detection method. In this study, a rapid nucleic acid of Escherichia coli O157:H7 was detected using rfbE gene based on CRISPR/Cas12a system. The limit of detection for DNA and bacterial concentrations was 0.9 pg/mu L and 6.5 x 104 CFU/mL, respectively. Furthermore, when the initial bacterial inoculum was 14 CFU/mL in the ground beef sample, the target was detected after 4 h of culture through Metal Organic Framework immunomagnetic beads (Fe3O4@PDA@UiO-66-NH2) enrichment. |
| WOS关键词 | METAL-ORGANIC FRAMEWORKS ; IN-GROUND BEEF ; DIAGNOSTICS ; ENRICHMENT |
| 资助项目 | National Key R&D Program of China[2018YFC1603900] |
| WOS研究方向 | Food Science & Technology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000684874200009 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/298002]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yang, Meicheng |
| 作者单位 | 1.Shanghai Inst Food & Drug Control, NMPA Key Lab Testing Technol Pharmaceut Microbiol, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shujuan,Fan, Yiling,Feng, Zhen,et al. Rapid nucleic acid detection of Escherichia coli O157:H7 based on CRISPR/ Cas12a system[J]. FOOD CONTROL,2021,130:8. |
| APA | Wang, Shujuan.,Fan, Yiling.,Feng, Zhen.,Song, Minghui.,Li, Qiongqiong.,...&Yang, Meicheng.(2021).Rapid nucleic acid detection of Escherichia coli O157:H7 based on CRISPR/ Cas12a system.FOOD CONTROL,130,8. |
| MLA | Wang, Shujuan,et al."Rapid nucleic acid detection of Escherichia coli O157:H7 based on CRISPR/ Cas12a system".FOOD CONTROL 130(2021):8. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。